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Further, cystic hydatidosis in farm animals causes severe economic losses. Although benzimidazole derivatives such as mebendazole and albendazole are being used as therapeutic agents, there is often no complete recovery after treatment. Hence, in searching for novel treatment options, we examined the in vitro efficacies of a number of isoflavones against\n            <jats:italic>Echinococcus<\/jats:italic>\n            metacestodes and protoscoleces. The most prominent isoflavone, genistein, exhibits significant metacestodicidal activity in vitro. However, genistein binds to the estrogen receptor and can thus induce estrogenic effects, which is a major concern during long-term chemotherapy. We have therefore investigated the activities of a number of synthetic genistein derivatives carrying a modified estrogen receptor binding site. One of these, Rm6423, induced dramatic breakdown of the structural integrity of the metacestode germinal layer of both species within 5 to 7 days of in vitro treatment. Further, examination of the culture medium revealed increased leakage of parasite proteins into the medium during treatment, but zymography demonstrated a decrease in the activity of metalloproteases. Moreover, two of the genistein derivatives, Rm6423 and Rm6426, induced considerable damage in\n            <jats:italic>E. granulosus<\/jats:italic>\n            protoscoleces, rendering them nonviable. These findings demonstrate that synthetic isoflavones exhibit distinct in vitro effects on\n            <jats:italic>Echinococcus<\/jats:italic>\n            metacestodes and protoscoleces, which could potentially be exploited further for the development of novel chemotherapeutical tools against larval-stage\n            <jats:italic>Echinococcus<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/aac.00578-06","type":"journal-article","created":{"date-parts":[[2006,10,25]],"date-time":"2006-10-25T20:58:50Z","timestamp":1161809930000},"page":"3770-3778","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":85,"title":["In Vitro Metacestodicidal Activities of Genistein and Other Isoflavones against\n            <i>Echinococcus multilocularis<\/i>\n            and\n            <i>Echinococcus granulosus<\/i>"],"prefix":"10.1128","volume":"50","author":[{"given":"Arunasalam","family":"Naguleswaran","sequence":"first","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Spicher","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Vonlaufen","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"Ortega-Mora","sequence":"additional","affiliation":[{"name":"Grupo SALUVET, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria s\/n, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Torgerson","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Zu\u0308rich, Winterthurstrasse 266a, CH-8057 Zu\u0308rich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Gottstein","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Hemphill","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse Faculty, University of Berne, La\u0308nggass-Strasse 122, CH-3012 Berne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"95","volume":"29","year":"1997","unstructured":"Adlercreutz, H., and W. 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