{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:22:44Z","timestamp":1771237364112,"version":"3.50.1"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI119788"],"award-info":[{"award-number":["AI119788"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,9,21]]},"abstract":"<jats:p>\n            Giardiasis, caused by the intestinal parasite\n            <jats:named-content content-type=\"genus-species\">Giardia lamblia<\/jats:named-content>\n            , is a severe diarrheal disease, endemic in poverty-stricken regions of the world, and also a common infection in developed countries. The available therapeutic options are associated with adverse effects, and\n            <jats:named-content content-type=\"genus-species\">G. lamblia<\/jats:named-content>\n            resistance to the standard-of-care drugs is spreading. Fumagillin, an antimicrosporidiosis drug, is a therapeutic agent with potential for the treatment of giardiasis. However, it exhibits considerable, albeit reversible, toxicity when used to treat immunocompromised microsporidiosis patients.\n          <\/jats:p>","DOI":"10.1128\/aac.00582-20","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T13:19:58Z","timestamp":1597065598000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Discovery and Preclinical Development of Antigiardiasis Fumagillol Derivatives"],"prefix":"10.1128","volume":"64","author":[{"given":"Janak","family":"Padia","sequence":"first","affiliation":[{"name":"Primetime Life Sciences, Germantown, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liudmila","family":"Kulakova","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey","family":"Galkin","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2823-7627","authenticated-orcid":false,"given":"Osnat","family":"Herzberg","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland, USA"},{"name":"Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.1.114-128.2001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-5-72"},{"issue":"7","key":"e_1_3_3_4_2","first-page":"11","article-title":"Giardiasis surveillance\u2014United States, 2003-2005","volume":"56","author":"Yoder JS","year":"2007","unstructured":"Yoder JS, Beach MJ, Centers for Disease Control and Prevention (CDC). 2007. Giardiasis surveillance\u2014United States, 2003-2005. MMWR Surveill Summ 56(SS-7):11\u201318.","journal-title":"MMWR Surveill Summ"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.02.015"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/320886"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-015-0051-1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.1.150-164.2001"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.03.004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00384-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1997.d01-132.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/02813430802602393"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/46.5.819"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b00143"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01159-06"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(88)90175-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(90)90025-h"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00618-10"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03834-14"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.115.2977.71"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2010.03166.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012924"},{"key":"e_1_3_3_23_2","volume-title":"European Medicines Agency pre-authorisation evaluation of medicines for human use","author":"EMEA","year":"2007","unstructured":"EMEA. 2007. Public summary of positive opinion for orphan designation of fumagillin for the treatment of diarrhoea associated with intestinal microsporidial infection. European Medicines Agency pre-authorisation evaluation of medicines for human use. EMEA, London, United Kingdom."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9394(14)73578-0"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/348555a0"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291x(91)91529-l"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.2174\/092986712799320709"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/dom.12457"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.117.246272"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.07.008"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.01.006"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5392.1324"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6099"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15183"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.can-04-0019"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19991102)38:21<3228::AID-ANIE3228>3.0.CO;2-8"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi051691k"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01227-4"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7714"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12357"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajhp\/56.6.547"},{"key":"e_1_3_3_42_2","first-page":"41","article-title":"Light- and temperature-induced degradation of fumagillin","volume":"73","author":"Agner G","year":"2003","unstructured":"Agner G, Gyorbiro A, Valko I, Vasvari-Debreczy L, Szabo A, Farkas G, Halasz J, Podanyi B, Brlik J, Simon K, Hermecz I. 2003. Light- and temperature-induced degradation of fumagillin. Acta Pharm Hung 73:41\u201345. (In Hungarian.)","journal-title":"Acta Pharm Hung"},{"key":"e_1_3_3_43_2","unstructured":"Farkas G Gyorbiro A Hermecz I Simon K Szabo A Vasvari A. May 2007. Stable fumagillin dicyclohexylamine salt used in veterinary medicine is reacted with an organic acid in alcoholic medium to free the salt. Patent no. EP 1230238 B1."},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1051\/apido:2004017"},{"key":"e_1_3_3_45_2","unstructured":"Abramovici B Dubois J-L. March 2003. Stable formulation containing fumagillin. US patent 6528539 B1."},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2003.10.008"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.112.048355"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0348-7139-6_7"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867053363540"},{"key":"e_1_3_3_50_2","first-page":"283","article-title":"Bioisosterism in drug design","volume":"21","author":"Lipinski CA","year":"1986","unstructured":"Lipinski CA. 1986. Bioisosterism in drug design. Annu Rep Med Chem 21:283\u2013291.","journal-title":"Annu Rep Med Chem"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.10570"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr950066q"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci0100806"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1039\/cs9790800563"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2006.02.006"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2009.07.029"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm901371v"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.2174\/1875397301206010093"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2758"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/16.5.589"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360100502"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.553123"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.1.162-170.2006"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00756522"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.1.2.175"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-x"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309110004665"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00582-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00582-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T23:36:06Z","timestamp":1645486566000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00582-20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,21]]},"references-count":66,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,9,21]]}},"alternative-id":["10.1128\/AAC.00582-20"],"URL":"https:\/\/doi.org\/10.1128\/aac.00582-20","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,21]]},"assertion":[{"value":"2020-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00582-20"}}