{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:38:47Z","timestamp":1792510727381,"version":"4.5.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Scientific Research Foundation of State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory","award":["2025XAKJ0100004"],"award-info":[{"award-number":["2025XAKJ0100004"]}]},{"name":"Guangdong Provincial Medical Research Foundation","award":["A2025209"],"award-info":[{"award-number":["A2025209"]}]},{"name":"Tertiary Education Scientific Research Project of Guangzhou Municipal Education Bureau","award":["2024312107"],"award-info":[{"award-number":["2024312107"]}]},{"name":"Zhongnanshan Medical Foundation of Guangdong Province","award":["ZNSXS-20260088"],"award-info":[{"award-number":["ZNSXS-20260088"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The global spread of carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      (CRPA) poses a critical clinical threat, with\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC<\/jats:sub>\n                      as a key mobile carbapenem resistance gene. Here, we investigated a novel\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      genetic element and its transmission in clinical ST2483 isolate PA100 via whole-genome sequencing, molecular, and phenotypic testing, and vesicle analysis. A unique 4,746-bp IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      -flanked structure inserted at the chromosomal\n                      <jats:italic toggle=\"yes\">InaA\/pncB2<\/jats:italic>\n                      locus was confirmed, which forms a circular transposition intermediate through IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      recombination. PA100 exhibited ~40-fold higher\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      copy number and ~25-fold higher mRNA expression vs chromosomal\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      strain PA3; meropenem exposure further elevated its transcription by nearly 70-fold, far exceeding controls. PA100 showed an extensively drug-resistant phenotype with high-level carbapenem resistance, while remaining susceptible to ceftazidime-avibactam and cefiderocol. No classical conjugation or transformation was observed, while intact outer membrane vesicles (OMVs) from PA100 carried\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      , supporting a non-plasmid, vesicle-associated route for transient delivery of the resistance gene under the tested conditions. The circular transposon remained genetically stable after 10 generations of passaging in PA100; however, stable maintenance in the PAO1 recipient was not detected after passage. In summary, we first report an IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      -driven circular\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      transposon in ST2483\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      , conferring robust overexpression and stable inheritance in the donor strain, with OMVs potentially facilitating its horizontal spread, although stable maintenance in recipient cells was not demonstrated under the current assay conditions. These data advance understanding of\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC<\/jats:sub>\n                      dissemination in CRPA and highlight clinical surveillance priorities for rare high-risk sequence types.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00582-26","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:42:36Z","timestamp":1787578956000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["IS\n                    <i>26<\/i>\n                    -mediated circular\n                    <i>\n                      bla\n                      <sub>KPC<\/sub>\n                    <\/i>\n                    transposon in ST2483\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0566-8220","authenticated-orcid":true,"given":"Mingxiao","family":"Chen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2969-6799","authenticated-orcid":true,"given":"Qingqing","family":"Zhi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/000b7ms85","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaoshan","family":"Luo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00zat6v61","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Basic Medicine, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6590-9774","authenticated-orcid":true,"given":"Meifang","family":"Lin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canbo","family":"Fan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoyan","family":"Zhong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Third Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Deng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwen","family":"Xiao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiang","family":"Zhu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qb7bg95","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Laboratory Medicine Department, The Second 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