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Currently, 30\u201350% of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      isolates are intrinsically resistant to amphotericin B. In this study, we characterized a clinical case of acquired amphotericin B resistance using whole-genome sequencing, a large-scale phenotypic screen, comprehensive sterol profiling, and genotypic reversion using CRISPR. Data obtained in this study provide evidence that a deletion resulting in a frameshift in\n                      <jats:italic toggle=\"yes\">ERG3<\/jats:italic>\n                      significantly contributes to the observed resistant phenotype, and a nonsense mutation in\n                      <jats:italic toggle=\"yes\">ERG4<\/jats:italic>\n                      may more modestly contribute to resistance. Characterization of this isolate also revealed that a fitness cost is associated with the abrogation of ergosterol production and its replacement with other late-stage sterols. This article presents a clinical case description of amphotericin B resistance from a frameshift mutation in\n                      <jats:italic toggle=\"yes\">ERG3<\/jats:italic>\n                      in\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      and marks an advancement in the understanding of antifungal resistance in this fungal pathogen.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00601-25","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:00:21Z","timestamp":1758542421000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Acquired amphotericin B resistance attributed to a mutated\n                    <i>ERG3<\/i>\n                    in\n                    <i>Candidozyma auris<\/i>"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7245-3182","authenticated-orcid":true,"given":"Lauryn","family":"Massic","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01keh0577","id-type":"ROR","asserted-by":"publisher"}],"name":"Nevada State Public Health Laboratory","place":["Reno, USA"]},{"id":[{"id":"https:\/\/ror.org\/01keh0577","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Nevada Reno School of Medicine","place":["Reno, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-9561","authenticated-orcid":true,"given":"Laura A.","family":"Doorley","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02r3e0967","id-type":"ROR","asserted-by":"publisher"}],"name":"St Jude Children's Research Hospital","place":["Memphis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Jones","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02r3e0967","id-type":"ROR","asserted-by":"publisher"}],"name":"St Jude Children's Research Hospital","place":["Memphis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Richardson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01keh0577","id-type":"ROR","asserted-by":"publisher"}],"name":"Nevada State Public Health Laboratory","place":["Reno, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Denise","family":"Siao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01keh0577","id-type":"ROR","asserted-by":"publisher"}],"name":"Nevada State Public Health Laboratory","place":["Reno, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Siao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01keh0577","id-type":"ROR","asserted-by":"publisher"}],"name":"Nevada State Public Health Laboratory","place":["Reno, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2158-3175","authenticated-orcid":true,"given":"Philip","family":"Dykema","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02x2akc96","id-type":"ROR","asserted-by":"publisher"}],"name":"Washington State Department of Health","place":["Olympia, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Hua","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02x2akc96","id-type":"ROR","asserted-by":"publisher"}],"name":"Washington State Department of Health","place":["Olympia, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Schneider","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02x2akc96","id-type":"ROR","asserted-by":"publisher"}],"name":"Washington State Department of Health","place":["Olympia, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina A.","family":"Cuomo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05gq02987","id-type":"ROR","asserted-by":"publisher"}],"name":"Brown University Division of Biology and Medicine","place":["Providence, USA"]},{"id":[{"id":"https:\/\/ror.org\/05a0ya142","id-type":"ROR","asserted-by":"publisher"}],"name":"Broad Institute","place":["Cambridge, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4401-0679","authenticated-orcid":true,"given":"P. 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