{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:36:45Z","timestamp":1790339805304,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter cloacae<\/jats:named-content>\n            is among the most important pathogens responsible for nosocomial infections and outbreaks. In this study, 77\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter<\/jats:named-content>\n            isolates were collected: 27 isolates from Algerian hospitals (in Constantine, Annaba, and Skikda) and 50 isolates from Marseille, France. All strains were identified by matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS). Antibiotic susceptibility testing was performed by the disk diffusion method. PCR was used to detect extended-spectrum-beta-lactamase (ESBL)-encoding, fluoroquinolone resistance-encoding, and aminoglycoside-modifying enzyme (AME) genes. Epidemiological typing was performed using MALDI-TOF MS with data mining approaches, along with multilocus sequence typing (MLST). Sixty-eight isolates (27 from Algeria, 41 from Marseille) were identified by MALDI-TOF MS as\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. cloacae<\/jats:named-content>\n            . Resistance to antibiotics in the Algerian isolates was significantly higher than that in the strains from Marseille, especially for beta-lactams and aminoglycosides. Eighteen of the 27 Algerian isolates and 11 of the 41 Marseille isolates possessed at least one ESBL-encoding gene:\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            and\/or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            . AME genes were detected in 20 of the 27 Algerian isolates and 8 of the 41 Marseille isolates [\n            <jats:italic>ant(2\u2033)<\/jats:italic>\n            -\n            <jats:italic>Ia<\/jats:italic>\n            ,\n            <jats:italic>aac(6\u2032)<\/jats:italic>\n            -\n            <jats:italic>Ib-cr<\/jats:italic>\n            ,\n            <jats:italic>aadA1<\/jats:italic>\n            ,\n            <jats:italic>aadA2<\/jats:italic>\n            , and\n            <jats:italic>armA<\/jats:italic>\n            ]. Conjugation experiments showed that\n            <jats:italic>armA<\/jats:italic>\n            was carried on a transferable plasmid. MALDI-TOF typing showed three separate clusters according to the geographical distribution and species level. An MLST-based phylogenetic tree showed a clade of 14\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. cloacae<\/jats:named-content>\n            isolates from a urology unit clustering together in the MALDI-TOF dendrogram, suggesting the occurrence of an outbreak in this unit. In conclusion, the ability of MALDI-TOF to biotype strains was confirmed, and surveillance measures should be implemented, especially for Algerian patients hospitalized in France.\n          <\/jats:p>","DOI":"10.1128\/aac.00615-15","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T02:44:26Z","timestamp":1438656266000},"page":"6477-6483","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["MALDI-TOF MS as a Tool To Detect a Nosocomial Outbreak of Extended-Spectrum-\u03b2-Lactamase- and ArmA Methyltransferase-Producing Enterobacter cloacae Clinical Isolates in Algeria"],"prefix":"10.1128","volume":"59","author":[{"given":"Nour Chems el Houda","family":"Khennouchi","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche sur les Maladies Infectieuses et Tropicales \u00c9mergentes (URMITE), UM63, CNRS 7278, IRD 198, INSERM 1095, IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Aix-Marseille-Universit\u00e9, Marseille, France"},{"name":"Laboratoire de Microbiologie et Biochimie Appliqu\u00e9e, D\u00e9partement de Biochimie, Facult\u00e9 des Sciences, Universit\u00e9 Badji Mokhtar, Annaba, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lotfi","family":"Loucif","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche sur les Maladies Infectieuses et Tropicales \u00c9mergentes (URMITE), UM63, CNRS 7278, IRD 198, INSERM 1095, IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Aix-Marseille-Universit\u00e9, Marseille, France"},{"name":"Laboratoire de Biotechnologie des Mol\u00e9cules Bioactives et de la Physiopathologie Cellulaire (LBMBPC), Universit\u00e9 El Hadj Lakhdar, Batna, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nafissa","family":"Boutefnouchet","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et Biochimie Appliqu\u00e9e, D\u00e9partement de Biochimie, Facult\u00e9 des Sciences, Universit\u00e9 Badji Mokhtar, Annaba, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamoudi","family":"Allag","sequence":"additional","affiliation":[{"name":"Etablissement Hospitalier Sp\u00e9cialis\u00e9 d'Uro-Nephrologie, Daksi, Constantine, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Rolain","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche sur les Maladies Infectieuses et Tropicales \u00c9mergentes (URMITE), UM63, CNRS 7278, IRD 198, INSERM 1095, IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Aix-Marseille-Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066358"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(02)00179-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/600885"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01811-12"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2013.10.008"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047636"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.162.2.185"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00789-13"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0161"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"382","DOI":"10.26719\/2012.18.4.382","article-title":"CTX-M-15 extended-spectrum beta-lactamases in Enterobacteriaceae in the intensive care unit of Tlemcen Hospital, Algeria","volume":"18","author":"Ahmed ZB","year":"2012","unstructured":"Ahmed ZB, Ayad A, Mesli E, Messai Y, Bakour R, Drissi M. 2012. 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