{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:45:09Z","timestamp":1791103509009,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The extracts and pure major constituents of Chios mastic gum (resin of\n            <jats:italic>Pistacia lentiscus<\/jats:italic>\n            var.\n            <jats:italic>chia<\/jats:italic>\n            ) were tested for their activities against\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            . A total mastic extract without polymer (TMEWP) was prepared after removal of the contained insoluble polymer in order to ameliorate solubility and enhance in vivo activity. Administration of TMEWP to\n            <jats:italic>H. pylori<\/jats:italic>\n            SS1-infected mice over the period of 3 months with an average dose of 0.75 mg\/day led to an approximately 30-fold reduction in the\n            <jats:italic>H. pylori<\/jats:italic>\n            colonization (1.5 log CFU\/g of tissue). However, no attenuation in the\n            <jats:italic>H. pylori<\/jats:italic>\n            -associated chronic inflammatory infiltration and the activity of chronic gastritis was observed. To further characterize potential active mastic constituents, the TMEWP was separated into an acidic and a neutral fraction. Both were extensively characterized by nuclear magnetic resonance and mass spectroscopy to elucidate the structure of the components contained within each fraction. After chromatographic separation, the acid fraction gave the major triterpenic acids, while the neutral fraction gave several triterpenic alcohols and aldehydes. Mastic extracts and isolated pure triterpenic acids were tested for in vitro activity against a panel of 11\n            <jats:italic>H. pylori<\/jats:italic>\n            clinical strains. The acid fraction was found to be the most active extract (minimum bactericidal concentration [MBC], 0.139 mg\/ml), and the most active pure compound was isomasticadienolic acid (MBC, 0.202 mg\/ml [0.443 mM]). Our results show that administration of TMEWP may be effective in reducing\n            <jats:italic>H. pylori<\/jats:italic>\n            colonization and that the major triterpenic acids in the acid extract may be responsible for such an activity.\n          <\/jats:p>","DOI":"10.1128\/aac.00642-06","type":"journal-article","created":{"date-parts":[[2006,11,21]],"date-time":"2006-11-21T01:47:59Z","timestamp":1164073679000},"page":"551-559","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":151,"title":["In Vitro and In Vivo Activities of Chios Mastic Gum Extracts and Constituents against\n            <i>Helicobacter pylori<\/i>"],"prefix":"10.1128","volume":"51","author":[{"given":"Sotirios","family":"Paraschos","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prokopios","family":"Magiatis","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Mitakou","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalliopi","family":"Petraki","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 11521, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonios","family":"Kalliaropoulos","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 11521, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petros","family":"Maragkoudakis","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 11521, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Mentis","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 11521, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionyssios","family":"Sgouras","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Hellenic Pasteur Institute, 127 Vassilisis Sofias Avenue, Athens 11521, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexios-Leandros","family":"Skaltsounis","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1984.tb00864.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(86)90165-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)81694-5"},{"key":"e_1_3_2_5_2","first-page":"1077","volume":"19","year":"2004","unstructured":"Assimopoulou, A. N., and V. P. Papageorgiou. 2004. GC-MS analysis of penta- and tetra-cyclic triterpenes from resins of Pistacia species. Part I. Pistacia lentiscus var. Chia. Biomed. Chromatogr.19:1077-1079.","journal-title":"Chia. Biomed. Chromatogr."},{"key":"e_1_3_2_6_2","first-page":"4150","volume":"189","year":"1956","unstructured":"Barton, D. H. R., and E. Seoane. 1956. Triterpenoids. Part XXII. The constitution and stereochemistry of masticadienonic acid. J. Chem. Soc.189:4150-4157.","journal-title":"J. Chem. Soc."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg366"},{"key":"e_1_3_2_8_2","first-page":"S49","volume":"96","year":"2001","unstructured":"Bona, S., L. Bono, L. Daghetta, and P. Marone. 2001. Bactericidal activity of Pistacia lentiscus gum mastic against Helicobacter pylori. Am. J. Gastroenterol.96:S49.","journal-title":"Am. J. Gastroenterol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(90)80135-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/np000129m"},{"key":"e_1_3_2_11_2","first-page":"1767","volume":"26","year":"1986","unstructured":"de Pascual Teresa, J., J. G. Urones, P. Basabe, M. J. Sexmero Cuadrado, and R. Fernandez Moro. 1986. Triterpens from Euphorbia broteri. Phytochemistry26:1767-1776.","journal-title":"Phytochemistry"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199610000-00001"},{"key":"e_1_3_2_13_2","first-page":"1946","volume":"339","year":"1998","unstructured":"Huwez, F. U., D. Thirlwell, A. Cockayne, and D. A. Ala'Aldeen. 1998. Mastic gum kills Helicobacter pylori. N. Engl. J. Med.339:1946.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/np010211x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.777"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.10.2623-2629.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70155-0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg057"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.772-774.1999"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/003655202317284165"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(91)80095-I"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2001.13.6.611"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)90421-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1868"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)89290-0"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0028-1097586"},{"key":"e_1_3_2_27_2","first-page":"4158","volume":"189","year":"1956","unstructured":"Seoane, E. 1956. Further crystalline constituents of gum mastic. J. Am. Chem. Soc.189:4158-4160.","journal-title":"J. Am. Chem. Soc."},{"key":"e_1_3_2_28_2","first-page":"1378","volume":"12","year":"2005","unstructured":"Sgouras, D. N., E. G. Panayotopoulou, B. Martinez-Gonzalez, K. Petraki, S. Michopoulos, and \u0391. Mentis. 2005 Lactobacillus johnsonii La1 attenuates Helicobacter pylori-associated gastritis and reduces the levels of proinflammatory chemokines in C57BL\/6 mice. Clin. Diagn. Lab. Immunol.12:1378-1386.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.1.518-526.2004"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(96)00266-X"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(91)80097-K"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00052-9"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0964-8305(95)00103-4"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(98)00228-7"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)92719-8"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(01)00633-3"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00642-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00642-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T19:14:19Z","timestamp":1645470859000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00642-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1128\/AAC.00642-06"],"URL":"https:\/\/doi.org\/10.1128\/aac.00642-06","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2]]},"assertion":[{"value":"2006-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}