{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:13:52Z","timestamp":1785406432937,"version":"3.56.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      We report the transposition of a 9,045-bp transposon (Tn\n                      <jats:italic toggle=\"yes\">552-II<\/jats:italic>\n                      ) carrying the\n                      <jats:italic toggle=\"yes\">tnpABC<\/jats:italic>\n                      \/target-gene-like(\n                      <jats:italic toggle=\"yes\">tgl<\/jats:italic>\n                      )\/\n                      <jats:italic toggle=\"yes\">blaZ-R1-I<\/jats:italic>\n                      array to a conjugative plasmid (pCtra) in pediatric multidrug-resistant community-associated methicillin-resistant\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      . Tn\n                      <jats:italic toggle=\"yes\">552-II<\/jats:italic>\n                      contained a 7-bp target site (\n                      <jats:italic toggle=\"yes\">att<\/jats:italic>\n                      ) on the left; an internal\n                      <jats:italic toggle=\"yes\">i-att<\/jats:italic>\n                      on the right, apparently \u201cbrought-in\u201d through a circular intermediate; and\n                      <jats:italic toggle=\"yes\">i-att2<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">i-att3<\/jats:italic>\n                      in the\n                      <jats:italic toggle=\"yes\">tgl<\/jats:italic>\n                      gene and its neighboring gene, respectively. This transposition enhanced ampicillin resistance and facilitated Tn\n                      <jats:italic toggle=\"yes\">552-II<\/jats:italic>\n                      spread at a frequency of 10\n                      <jats:sup>\u22123<\/jats:sup>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00647-25","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T13:00:59Z","timestamp":1756904459000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Transposition of Tn\n                    <i>552-II<\/i>\n                    (a Tn\n                    <i>552<\/i>\n                    derivative) to the conjugative pCtra plasmid family in pediatric multidrug-resistant community-associated MRSA"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2148-9514","authenticated-orcid":true,"given":"Tsai-Wen","family":"Wan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053a6xa29","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Epidemiology, Genomics, and Evolution, International Medical Education and Research Center","place":["Niigata, Japan"]},{"id":[{"id":"https:\/\/ror.org\/05bqach95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine","place":["Taipei City, Taiwan"]},{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University","place":["Kaohsiung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Shintani","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01w6wtk13","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Environment and Energy Systems, Graduate School of Science and Technology, Shizuoka University","place":["Hamamatsu, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji K.","family":"Kojima","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01jngdt03","id-type":"ROR","asserted-by":"publisher"}],"name":"Genetic Information Research Institute (GIRI)","place":["Cupertino, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiko","family":"Moriguchi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05pp6zn13","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatrics, Kaizuka City Hospital","place":["Kaizuka, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Chun","family":"Hung","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University","place":["Kaohsiung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vxgjw72","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare","place":["Taipei, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee-Jene","family":"Teng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bqach95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine","place":["Taipei City, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0920-271X","authenticated-orcid":true,"given":"Tatsuo","family":"Yamamoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053a6xa29","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Epidemiology, Genomics, and Evolution, International Medical Education and Research Center","place":["Niigata, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"World Health Organization. 2023. 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Churchward G. 2002. Conjugative transposons and related mobile elements, p 177-191. In Craig NL, Craigie R, Gellert M, Lambowitz AM (ed), Mobile DNA II. ASM Press, American Society for Microbiology, Washington, DC"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0271627"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-023-00295-5"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab210"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00647-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00647-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T13:02:57Z","timestamp":1759323777000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00647-25"}},"subtitle":[],"editor":[{"given":"Boudewijn L.","family":"de Jonge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1128\/aac.00647-25"],"URL":"https:\/\/doi.org\/10.1128\/aac.00647-25","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-04-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00647-25"}}