{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:17:22Z","timestamp":1788369442825,"version":"build-2803163510"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U25A2088, 82360623"],"award-info":[{"award-number":["U25A2088, 82360623"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["AB24010134"],"award-info":[{"award-number":["AB24010134"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This study aimed to establish epidemiological cutoff values (ECVs) for amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, and isavuconazole against\n                      <jats:italic toggle=\"yes\">Talaromyces marneffei<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">T. marneffei<\/jats:italic>\n                      ) using the CLSI broth microdilution method. Antifungal susceptibility testing of 207\n                      <jats:italic toggle=\"yes\">T. marneffei<\/jats:italic>\n                      isolates was performed independently at five laboratories across China according to CLSI M27-A4 guidelines. Minimum inhibitory concentration (MIC) distributions were analyzed using ECOFFinder software (version 2.1), and ECVs were set at the upper MIC limit encompassing 97.5% of the modeled wild-type (WT) population. ECVs were determined as follows: amphotericin B (AMB) 8 \u00b5g\/mL, voriconazole (VRC) 0.03 \u00b5g\/mL, itraconazole (ITC) 0.125 \u00b5g\/mL, posaconazole (POS) 0.06 \u00b5g\/mL, isavuconazole (ISA) 0.125 \u00b5g\/mL, and fluconazole (FLC) 8 \u00b5g\/mL. MIC distributions for all six agents conformed well to the fitted models (normal or log-normal), supporting the validity of ECV determination. No non-WT isolates were detected for amphotericin B, whereas non-WT rates for azoles were 15.5% (voriconazole), 8.45% (itraconazole), 6.37% (posaconazole), 3.0% (fluconazole), and 1.02% (isavuconazole). Among voriconazole non-WT isolates, all were from patients with prior voriconazole exposure. This study provides the first standardized ECVs for\n                      <jats:italic toggle=\"yes\">T. marneffei<\/jats:italic>\n                      against six key antifungal agents. The data establish a reference framework for antifungal susceptibility testing and resistance surveillance and highlight the need for continued monitoring of azole susceptibility in this pathogen.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00650-26","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:43:14Z","timestamp":1785937394000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of epidemiological cutoff values for yeast-phase\n                    <i>Talaromyces marneffei<\/i>\n                    against six antifungal agents: a multicenter study in China"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8328-3776","authenticated-orcid":false,"given":"Kai-su","family":"Pan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/030sc3x20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Dermatology, The First Affiliated Hospital of Guangxi Medical University","place":["Nanning, China"]},{"name":"Guangxi Scientific and Technological Innovation Cooperation Base of Mycosis Prevention and Control","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1644-6942","authenticated-orcid":true,"given":"Ying-gai","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02z1vqm45","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Dermatology and Venerology, Peking University First Hospital","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Lu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01px77p81","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5148-2922","authenticated-orcid":true,"given":"Xiao-fang","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02j6cz137","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Mycology, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College","place":["Nanjing, 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