{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:07:57Z","timestamp":1779887277155,"version":"3.53.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Resistance to extended-spectrum cephalosporins (ESC) among members of the family\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            occurs worldwide; however, little is known about ESC resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            strains from companion animals. Clinical isolates of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            were collected from veterinary diagnostic laboratories throughout the United States from 2008 to 2009.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            isolates (\n            <jats:italic>n<\/jats:italic>\n            = 54) with reduced susceptibility to ceftazidime or cefotaxime (MIC \u2265 16 \u03bcg\/ml) and extended-spectrum-\u03b2-lactamase (ESBL) phenotypes were analyzed. PCR and sequencing were used to detect mutations in ESBL-encoding genes and the regulatory region of the chromosomal gene\n            <jats:italic>ampC<\/jats:italic>\n            . Conjugation experiments and plasmid identification were conducted to examine the transferability of resistance to ESCs. All isolates carried the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-1<\/jats:sub>\n            -group \u03b2-lactamase genes in addition to one or more of the following \u03b2-lactamase genes:\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-3<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CMY-2<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-14-like<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-1.<\/jats:sub>\n            Different\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            sequence variants were detected in some isolates (\n            <jats:italic>n<\/jats:italic>\n            = 40). Three isolates harbored a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM-181<\/jats:sub>\n            gene with a novel mutation resulting in an Ala184Val substitution. Approximately 78% of the isolates had mutations in promoter\/attenuator regions of the chromosomal gene\n            <jats:italic>ampC<\/jats:italic>\n            , one of which was a novel insertion of adenine between bases \u221228 and \u221229. Plasmids ranging in size from 11 to 233 kbp were detected in the isolates, with a common plasmid size of 93 kbp identified in 60% of isolates. Plasmid-mediated transfer of \u03b2-lactamase genes increased the MICs (\u226516-fold) of ESCs for transconjugants. Replicon typing among isolates revealed the predominance of IncI and IncFIA plasmids, followed by IncFIB plasmids. This study shows the emergence of conjugative plasmid-borne ESBLs among\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            strains from companion animals in the United States, which may compromise the effective therapeutic use of ESCs in veterinary medicine.\n          <\/jats:p>","DOI":"10.1128\/aac.00656-11","type":"journal-article","created":{"date-parts":[[2011,9,27]],"date-time":"2011-09-27T03:50:46Z","timestamp":1317095446000},"page":"5666-5675","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Molecular Characterization of Resistance to Extended-Spectrum Cephalosporins in Clinical Escherichia coli Isolates from Companion Animals in the United States"],"prefix":"10.1128","volume":"55","author":[{"given":"Bashar W.","family":"Shaheen","sequence":"first","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Nayak","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L.","family":"Foley","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ohgew","family":"Kweon","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Deck","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miseon","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Rafii","sequence":"additional","affiliation":[{"name":"Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn M.","family":"Boothe","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki370"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.3.1262-1264.2005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.10.3156-3163.2002"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.833-835.2005"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.6.783"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2308-2313.2004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.4.447"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(03)00063-4"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4745-4750.2005"},{"key":"e_1_3_3_14_2","volume-title":"Approved standard M31-A3.","author":"CLSI.","year":"2008","unstructured":"CLSI. 2008. 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