{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:12:35Z","timestamp":1791421955545,"version":"4.3.3"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Trimethoprim-sulfamethoxazole (co-trimoxazole) is the primary drug used for oral eradication therapy of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Burkholderia pseudomallei<\/jats:named-content>\n            infections (melioidosis). Here, we demonstrate that trimethoprim resistance is widespread in clinical and environmental isolates from northeast Thailand and northern Australia. This resistance was shown to be due to BpeEF-OprC efflux pump expression. No dihydrofolate reductase target mutations were involved, although frequent insertion of IS\n            <jats:italic>Bma<\/jats:italic>\n            2 was noted within the putative\n            <jats:italic>folA<\/jats:italic>\n            transcriptional terminator. All isolates tested remained susceptible to trimethoprim-sulfamethoxazole, suggesting that resistance to trimethoprim alone in these strains probably does not affect the efficacy of co-trimoxazole therapy.\n          <\/jats:p>","DOI":"10.1128\/aac.00660-13","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T01:07:13Z","timestamp":1372727233000},"page":"4381-4386","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["The BpeEF-OprC Efflux Pump Is Responsible for Widespread Trimethoprim Resistance in Clinical and Environmental Burkholderia pseudomallei Isolates"],"prefix":"10.1128","volume":"57","author":[{"given":"Nicole L.","family":"Podnecky","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA"},{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[{"name":"Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert P.","family":"Schweizer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA"},{"name":"Rocky Mountain Regional Center of Excellence for Biodefense and Emerging Infectious Diseases Research, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(08)70002-6"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204699"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1385"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000900"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldr007"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.4.3.445"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.116"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1407.071501"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1812.120638"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817886"},{"key":"e_1_3_2_13_2","first-page":"856","article-title":"Comparison between the antimicrobial susceptibility of Burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination","volume":"83","author":"Lumbiganon P","year":"2000","unstructured":"LumbiganonPTattawasatraUChetchotisakdPWongratanacheewinSThinkamropB. 2000. Comparison between the antimicrobial susceptibility of Burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination. J. Med. Assoc. Thai. 83:856\u2013860.","journal-title":"J. Med. Assoc. Thai."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(02)00016-X"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki151"},{"key":"e_1_3_2_16_2","first-page":"174","article-title":"Cases of melioidosis in a university teaching hospital in Malaysia","volume":"41","author":"Raja NS","year":"2008","unstructured":"RajaNS. 2008. Cases of melioidosis in a university teaching hospital in Malaysia. J. Microbiol. Immunol. Infect. 41:174\u2013179.","journal-title":"J. Microbiol. Immunol. 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