{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:36:34Z","timestamp":1791200194780,"version":"4.2.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Fluoroquinolones are among the drugs most extensively used for the treatment of bacterial infections in human and veterinary medicine. Resistance to quinolones can be chromosome or plasmid mediated. The chromosomal mechanism of resistance is associated with mutations in the DNA gyrase- and topoisomerase IV-encoding genes and mutations in regulatory genes affecting different efflux systems, among others. We studied the role of the acquisition of a mutation in the\n            <jats:italic>gyrA<\/jats:italic>\n            gene in the virulence and protein expression of uropathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            (UPEC). The HC14366M strain carrying a mutation in the\n            <jats:italic>gyrA<\/jats:italic>\n            gene (S83L) was found to lose the capacity to cause cystitis and pyelonephritis mainly due to a decrease in the expression of the\n            <jats:italic>fimA<\/jats:italic>\n            ,\n            <jats:italic>papA<\/jats:italic>\n            ,\n            <jats:italic>papB<\/jats:italic>\n            , and\n            <jats:italic>ompA<\/jats:italic>\n            genes. The levels of expression of the\n            <jats:italic>fimA<\/jats:italic>\n            ,\n            <jats:italic>papB<\/jats:italic>\n            , and\n            <jats:italic>ompA<\/jats:italic>\n            genes were recovered on complementing the strain with a plasmid containing the\n            <jats:italic>gyrA<\/jats:italic>\n            wild-type gene. However, only a slight recovery was observed in the colonization of the bladder in the GyrA complement strain compared to the mutant strain in a murine model of ascending urinary tract infection. In conclusion, a mutation in the\n            <jats:italic>gyrA<\/jats:italic>\n            gene of uropathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            reduced the virulence of the bacteria, likely in association with the effect of DNA supercoiling on the expression of several virulence factors and proteins, thereby decreasing their capacity to cause cystitis and pyelonephritis.\n          <\/jats:p>","DOI":"10.1128\/aac.00665-15","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T15:24:53Z","timestamp":1432740293000},"page":"4662-4668","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Effects of a Mutation in the\n            <i>gyrA<\/i>\n            Gene on the Virulence of Uropathogenic Escherichia coli"],"prefix":"10.1128","volume":"59","author":[{"given":"Javier","family":"S\u00e1nchez-C\u00e9spedes","sequence":"first","affiliation":[{"name":"Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Roc\u00edo\/CSIC\/University of Seville, Unit of Infectious Diseases, Microbiology and Preventive Medicine, Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"S\u00e1ez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Frimodt-M\u00f8ller","sequence":"additional","affiliation":[{"name":"University Hospital, Department of Clinical Microbiology, Hvidovre, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Vila","sequence":"additional","affiliation":[{"name":"ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain"},{"name":"Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara M.","family":"Soto","sequence":"additional","affiliation":[{"name":"ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2008.11.001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/314056"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)07322-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70599-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00339-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.2.491"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/344789a0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/342955"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2962-2964.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01740-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.2.649-653.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh505"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/318855"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4572-4579.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.3.625"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.08.019"},{"key":"e_1_3_2_18_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 17th informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. 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