{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T11:03:47Z","timestamp":1769166227816,"version":"3.49.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Very little is known about the prevalence and composition of various types of extended-spectrum \u03b2-lactamases (ESBL) in pediatric patients. The aims of this study were the following: (i) to determine the prevalence of ESBLs among\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            in a tertiary-care pediatric population; (ii) to characterize the genetic composition of the identified ESBL enzymes; and (iii) to determine the relative prevalence of CTX-M enzymes and\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            ST131 strains among ESBL-producing isolates in the same pediatric patient population. Among the 1,430\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            isolates screened for elevated MICs to cefotaxime and\/or ceftazidime from pediatric patients during a 1-year period, 94 isolates possessed at least one ESBL gene. CTX-M was the most commonly isolated ESBL type, consisting of 74% of all ESBLs versus 27% TEM and 24% SHV enzymes. Sequence analysis and probe-specific real-time PCR revealed that the majority (80%) of the CTX-M-type ESBLs were CTX-M-15 enzymes, followed by CTX-M-14 (17%) and CTX-M-27(2.8%). Multilocus sequence typing (MLST) and repetitive PCR analyses revealed that the relative prevalence of ST131 among ESBL-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            isolates is 10.2%. This study highlights the growing problem of ESBL resistance in pediatric\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            isolates and demonstrates a transition toward the predominance of CTX-M-type enzymes among ESBL-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            organisms causing pediatric infections.\n          <\/jats:p>","DOI":"10.1128\/aac.00666-12","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T07:48:33Z","timestamp":1340783313000},"page":"4765-4770","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Prevalence and Molecular Characterization of Extended-Spectrum-\u03b2-Lactamase-Producing Enterobacteriaceae in a Pediatric Patient Population"],"prefix":"10.1128","volume":"56","author":[{"given":"Lakshmi","family":"Chandramohan","sequence":"first","affiliation":[{"name":"Departments of Pathology, Baylor College of Medicine, Houston, Texas, USA"},{"name":"Department of Pathology, Texas Children's Hospital, Houston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula A.","family":"Revell","sequence":"additional","affiliation":[{"name":"Departments of Pathology, Baylor College of Medicine, Houston, Texas, USA"},{"name":"Pediatrics, Baylor College of Medicine, Houston, Texas, USA"},{"name":"Department of Pathology, Texas Children's Hospital, Houston, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/jp.2008.73","article-title":"Extended-spectrum beta-lactamase producing Klebsiella pneumoniae in neonatal intensive care unit","volume":"28","author":"Abdel-Hady H","year":"2008","unstructured":"Abdel-HadyH HawasS El-DakerM El-KadyR. 2008. 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