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SA-17 is markedly less cytostatic than the parent compound, resulting in a selectivity index value of \u223c100. SA-17 also inhibits yellow fever virus 17D (YFV-17D) replication (EC\n            <jats:sub>50<\/jats:sub>\n            = 3.1 \u00b1 1.0 \u03bcg\/ml [4.8 \u00b1 1.5 \u03bcM]), although less efficiently than DENV replication, but proved inactive against a variety of enveloped and nonenveloped viruses. SA-17 inhibits\n            <jats:italic>in vitro<\/jats:italic>\n            flavivirus replication in a dose-dependent manner, as was assessed by virus yield reduction assays and quantification of viral RNA by means of real-time quantitative reverse transcriptase PCR (RT-qPCR) (\u223c2 to 3 log reduction). The anti-DENV activity was confirmed using a\n            <jats:italic>Renilla<\/jats:italic>\n            luciferase-expressing dengue reporter virus. Time-of-drug-addition studies revealed that SA-17 acts at the very early stages of the viral replication cycle (i.e., virus attachment and\/or virus entry). This observation was corroborated by the observation that SA-17, unlike the nucleoside analogue ribavirin, does not inhibit the replication of DENV subgenomic replicons. Preincubation of high-titer stocks of DENV or YFV-17D with \u22655 \u03bcg\/ml SA-17 resulted in 100% inhibition of viral infectivity (\u22653 log reduction). SA-17, however, did not prove virucidal.\n          <\/jats:p>","DOI":"10.1128\/aac.00686-10","type":"journal-article","created":{"date-parts":[[2010,9,14]],"date-time":"2010-09-14T04:59:47Z","timestamp":1284440387000},"page":"5269-5280","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["A Derivate of the Antibiotic Doxorubicin Is a Selective Inhibitor of Dengue and Yellow Fever Virus Replication\n            <i>In Vitro<\/i>"],"prefix":"10.1128","volume":"54","author":[{"given":"Suzanne J. 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