{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:24:44Z","timestamp":1790061884656,"version":"4.0.1"},"reference-count":80,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Programa de Apoio ao Desenvolvimento Cient\u00edfico da Faculdade de Ci\u00eancias Farmac\u00eauticas da UNESP"},{"DOI":"10.13039\/501100003593","name":"MCTI | Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/08248-1"],"award-info":[{"award-number":["2013\/08248-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Leishmaniasis is a disease found throughout the (sub)tropical parts of the world caused by protozoan parasites of the\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            genus. Despite the numerous problems associated with existing treatments, pharmaceutical companies continue to neglect the development of better ones. The high toxicity of current drugs combined with emerging resistance makes the discovery of new therapeutic alternatives urgent. We report here the evaluation of a binuclear cyclopalladated complex containing Pd(II) and\n            <jats:italic>N<\/jats:italic>\n            ,\n            <jats:italic>N<\/jats:italic>\n            \u2032-dimethylbenzylamine (Hdmba) against\n            <jats:named-content content-type=\"genus-species\">Leishmania amazonensis<\/jats:named-content>\n            . The compound [Pd(dmba)(\u03bc-N\n            <jats:sub>3<\/jats:sub>\n            )]\n            <jats:sub>2<\/jats:sub>\n            (CP2) inhibits promastigote growth (50% inhibitory concentration [IC\n            <jats:sub>50<\/jats:sub>\n            ] = 13.2 \u00b1 0.7 \u03bcM) and decreases the proliferation of intracellular amastigotes in\n            <jats:italic>in vitro<\/jats:italic>\n            incubated macrophages (IC\n            <jats:sub>50<\/jats:sub>\n            = 10.2 \u00b1 2.2 \u03bcM) without a cytotoxic effect when tested against peritoneal macrophages (50% cytotoxic concentration = 506.0 \u00b1 10.7 \u03bcM). In addition, CP2 was also active against\n            <jats:named-content content-type=\"genus-species\">T. cruzi<\/jats:named-content>\n            intracellular amastigotes (IC\n            <jats:sub>50<\/jats:sub>\n            = 2.3 \u00b1 0.5 \u03bcM, selective index = 225), an indication of its potential for use in Chagas disease therapy.\n            <jats:italic>In vivo<\/jats:italic>\n            assays using\n            <jats:named-content content-type=\"genus-species\">L. amazonensis<\/jats:named-content>\n            -infected BALB\/c showed an 80% reduction in parasite load compared to infected and nontreated animals. Also, compared to amphotericin B treatment, CP2 did not show any side effects, which was corroborated by the analysis of plasma levels of different hepatic and renal biomarkers. Furthermore, CP2 was able to inhibit\n            <jats:named-content content-type=\"genus-species\">Leishmania donovani<\/jats:named-content>\n            topoisomerase 1B (\n            <jats:italic>Ld<\/jats:italic>\n            topo1B), a potentially important target in this parasite. (This study has been registered at ClinicalTrials.gov under identifier NCT02169141.)\n          <\/jats:p>","DOI":"10.1128\/aac.00688-17","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:50:18Z","timestamp":1494895818000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Efficacy of a Binuclear Cyclopalladated Compound Therapy for Cutaneous Leishmaniasis in the Murine Model of Infection with Leishmania amazonensis and Its Inhibitory Effect on Topoisomerase 1B"],"prefix":"10.1128","volume":"61","author":[{"given":"Angela Maria Arenas","family":"Vel\u00e1squez","sequence":"first","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"},{"name":"S\u00e3o Paulo State University (UNESP), Institute of Chemistry, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willian Campos","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vutey","family":"Venn","sequence":"additional","affiliation":[{"name":"University of Rome, Tor Vergata, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Castelli","sequence":"additional","affiliation":[{"name":"University of Rome, Tor Vergata, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Santoro de","family":"Camargo","sequence":"additional","affiliation":[{"name":"University of Rome, Tor Vergata, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Pires","family":"de Assis","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Alves","family":"de Souza","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), Institute of Chemistry, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Rimoldi","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Campinas State University (UNICAMP), Biology Institute, Campinas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Gaban","family":"Passalacqua","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"},{"name":"S\u00e3o Paulo State University (UNESP), Institute of Chemistry, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Aristeu","family":"da Rosa","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Martins","family":"Baviera","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Eduardo","family":"Mauro","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), Institute of Chemistry, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Desideri","sequence":"additional","affiliation":[{"name":"University of Rome, Tor Vergata, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmo Eduardo","family":"Almeida-Amaral","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia A. S.","family":"Graminha","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67629-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(12)60084-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjp.2014.07.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/np300077r"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000818"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.03.005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200212000-00007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-004-0348-9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks275"},{"key":"e_1_3_2_11_2","volume-title":"Control of the leishmaniases: report of a meeting of the WHO Expert Committee on the Control of Leishmaniasis","author":"World Health Organization","year":"2010","unstructured":"World Health Organization. 2010. Control of the leishmaniases: report of a meeting of the WHO Expert Committee on the Control of Leishmaniasis. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/lds031"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(84)90501-X"},{"key":"e_1_3_2_14_2","first-page":"41","article-title":"In vitro activity of organometallic complexes of iridium, platinum, and rhodium on Trypanosoma b. gambiense, T. b. rhodesiense, and T. b. brucei","volume":"42","author":"Zinsstag J","year":"1991","unstructured":"Zinsstag J BrunR CraciunescuDG Parrondo IglesiasE. 1991. In vitro activity of organometallic complexes of iridium, platinum, and rhodium on Trypanosoma b. gambiense, T. b. rhodesiense, and T. b. brucei. Trop Med Parasitol42:41\u201344.","journal-title":"Trop Med Parasitol"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389557043487493"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2008.05.010"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2010.10.005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00323-10"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001626"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-009-0558-9"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.10.008"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2011.02.007"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2012.10.007"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.03.078"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-010-0006-3"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.2174\/138955751503150312120912"},{"key":"e_1_3_2_27_2","first-page":"257","article-title":"A type 1 DNA topoisomerase from the kinetoplast hemoflagellate Leishmania donovani","volume":"30","author":"Chakraborty AK","year":"1993","unstructured":"Chakraborty AK GuptaA MajumderHK. 1993. A type 1 DNA topoisomerase from the kinetoplast hemoflagellate Leishmania donovani. Indian J Biochem Biophys30:257\u2013263.","journal-title":"Indian J Biochem Biophys"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1983.tb07592.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1330762100"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.13.2745"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.01.022"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/205698a0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm0512241"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2007.12.005"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-40421999000300008"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2012.08.024"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2009.02.011"},{"key":"e_1_3_2_38_2","first-page":"24","article-title":"The activity of platinum, iridium, and rhodium drug complexes against Leishmania donovani","volume":"43","author":"Croft SL","year":"1992","unstructured":"Croft SL NealRA CraciunescuDG CertadfombonaG. 1992. The activity of platinum, iridium, and rhodium drug complexes against Leishmania donovani. Trop Med Parasitol43:24\u201328.","journal-title":"Trop Med Parasitol"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1159\/000056121"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1155\/MBD.2002.329"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2014.02.046"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11434"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2010.07.057"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2009.06.032"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1080\/00958972.2012.673718"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-50532007000800004"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1039\/b819061e"},{"key":"e_1_3_2_48_2","first-page":"1032","article-title":"Antiprotozoal activity of the cyclopalladated complexes against Leishmania amazonensis and Trypanosoma cruzi","volume":"27","author":"Vel\u00e1squez AM","year":"2016","unstructured":"Vel\u00e1squez AM De SouzaRA PassalacquaTG RibeiroAR ScontriM ChinCM De AlmeidaL Del CistiaML Da RosaJA MauroAE GraminhaMA. 2016. Antiprotozoal activity of the cyclopalladated complexes against Leishmania amazonensis and Trypanosoma cruzi. J Braz Chem Soc27:1032\u20131039.","journal-title":"J Braz Chem Soc"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4683"},{"key":"e_1_3_2_50_2","first-page":"1","volume-title":"The leishmaniases in biology and medicine","author":"Lainson R","year":"1987","unstructured":"Lainson R ShawJJ. 1987. Evolution, classification, and geographical distribution, p 1\u2013120. InKillick-KendrickR PetersW (ed), The leishmaniases in biology and medicine. Academic Press, London, United Kingdom."},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.10-0171"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.18181"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci300367a"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci5000467"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80081-0"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2014.07.006"},{"key":"e_1_3_2_57_2","first-page":"6318","article-title":"Expression of human DNA topoisomerase I in yeast cells lacking yeast DNA topoisomerase I: restoration of sensitivity of the cells to the antitumor drug camptothecin","volume":"49","author":"Bjornsti MA","year":"1989","unstructured":"Bjornsti MA BenedettiP VigliantiGA WangJC. 1989. Expression of human DNA topoisomerase I in yeast cells lacking yeast DNA topoisomerase I: restoration of sensitivity of the cells to the antitumor drug camptothecin. Cancer Res49:6318\u20136323.","journal-title":"Cancer Res"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(02)00297-8"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3347"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.02.024"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.004416"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2007.08.020"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01006a012"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0277-5387(98)00310-6"},{"key":"e_1_3_2_65_2","first-page":"191","article-title":"Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco","volume":"20","author":"Silva LH","year":"1953","unstructured":"Silva LH NussenzweigV. 1953. Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco. Folia Clin Biol20:191\u2013208.","journal-title":"Folia Clin Biol"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1089\/152791602321105799"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2015.05.072"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00052-14"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(85)90108-2"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00918753"},{"key":"e_1_3_2_71_2","first-page":"80","article-title":"Strain of Leishmania donovani Host resistance to genus Leishmania","volume":"45","author":"Stauber LA","year":"1958","unstructured":"Stauber LA . 1958. Strain of Leishmania donovani Host resistance to genus Leishmania. Rice Inst Pam45:80\u201396.","journal-title":"Rice Inst Pam"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00273-15"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.8.2513-2517.2003"},{"key":"e_1_3_2_74_2","doi-asserted-by":"crossref","unstructured":"Mullen\nAB BaillieAJ CarterKC. 1998. Visceral leishmaniasis in the BALB\/c mouse: a comparison of the efficacy of a nonionic surfactant formulation of sodium stibogluconate with those of three proprietary formulations of amphotericin B. Antimicrob Agents Chemother42:2722\u20132725. http:\/\/aac.asm.org\/content\/42\/10\/2722.long.","DOI":"10.1128\/AAC.42.10.2722"},{"key":"e_1_3_2_75_2","first-page":"39","article-title":"Provisional recommendation on IFCC methods for the measurement of catalytic concentration of enzymes. 2. IFCC method for aspartate aminotransferase","volume":"15","author":"Bergmeyer HU","year":"1977","unstructured":"Bergmeyer HU BowersGN HorderM MossDW. 1977. Provisional recommendation on IFCC methods for the measurement of catalytic concentration of enzymes. 2. IFCC method for aspartate aminotransferase. J Clin Chem Clin Biochem15:39\u201351.","journal-title":"J Clin Chem Clin Biochem"},{"key":"e_1_3_2_76_2","first-page":"569","article-title":"Serum glutamic pyruvic transaminase in cardiac and hepatic disease","volume":"91","author":"Wr\u00f3blewski F","year":"1956","unstructured":"Wr\u00f3blewski F LadueJS. 1956. Serum glutamic pyruvic transaminase in cardiac and hepatic disease. PSEBM91:569\u2013571.","journal-title":"PSEBM"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365517409082499"},{"key":"e_1_3_2_78_2","doi-asserted-by":"crossref","unstructured":"Perry\nBW DoumasBT BayseDD ButlerT CohenA FellowsW GarberCC HowellB KochT KrishnamurthyS LouderbackA McCombRB MillerD MillerRR RandRN SchafferR. 1983. A candidate reference method for determination of bilirubin in serum: test for transferability. Clin Chem29:297\u2013301. http:\/\/www.clinchem.org\/content\/29\/2\/297.long.","DOI":"10.1093\/clinchem\/29.2.297"},{"key":"e_1_3_2_79_2","doi-asserted-by":"crossref","unstructured":"Doumas\nBT Poon Pat Kwok-Cheung PerryBW. 1985. Candidate reference method for determination of total bilirubin in serum: development and validation. Clin Chem31:1779\u20131789. http:\/\/www.clinchem.org\/content\/31\/11\/1779.long.","DOI":"10.1093\/clinchem\/31.11.1779"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(71)90452-9"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-395630-9.50082-7"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00688-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00688-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:04:34Z","timestamp":1645477474000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00688-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":80,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1128\/AAC.00688-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.00688-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]},"assertion":[{"value":"2017-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00688-17"}}