{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:21:05Z","timestamp":1791238865800,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>bc<\/jats:italic>\n            <jats:sub>1<\/jats:sub>\n            complex is central to mitochondrial bioenergetics and the target of the antimalarial drug atovaquone that binds in the quinol oxidation (Q\n            <jats:sub>o<\/jats:sub>\n            ) site of the complex. Structural analysis has shown that the Q\n            <jats:sub>o<\/jats:sub>\n            site residue Y279 (Y268 in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Plasmodium falciparum<\/jats:named-content>\n            ) is key for atovaquone binding. Consequently, atovaquone resistance can be acquired by mutation of that residue. In addition to the probability of amino acid substitution, the level of atovaquone resistance and the loss of\n            <jats:italic>bc<\/jats:italic>\n            <jats:sub>1<\/jats:sub>\n            complex activity that are associated with the novel amino acid would restrict the nature of resistance-driven mutations occurring on atovaquone exposure in native parasite populations. Using the yeast model, we characterized the effect of all the amino acid replacements resulting from a single nucleotide substitution at codon 279: Y279C, Y279D, Y279F, Y279H, Y279N, and Y279S (Y279C, D, F, H, N, and S). Two residue changes that required a double nucleotide substitution, Y279A and W, were added to the series. We found that mutations Y279A, C, and S conferred high atovaquone resistance but decreased the catalytic activity. Y279F had wild-type enzymatic activity and sensitivity to atovaquone, while the other substitutions caused a dramatic respiratory defect. The results obtained with the yeast model were examined in regard to atomic structure and compared to the reported data on the evolution of acquired atovaquone resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. falciparum<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.00710-15","type":"journal-article","created":{"date-parts":[[2015,4,28]],"date-time":"2015-04-28T01:51:47Z","timestamp":1430185907000},"page":"4053-4058","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Saccharomyces cerevisiae-Based Mutational Analysis of the\n            <i>bc<\/i>\n            <sub>1<\/sub>\n            Complex Q\n            <sub>o<\/sub>\n            Site Residue 279 To Study the Trade-Off between Atovaquone Resistance and Function"],"prefix":"10.1128","volume":"59","author":[{"given":"Zehua","family":"Song","sequence":"first","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris-Sud, Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Clain","sequence":"additional","affiliation":[{"name":"UMR 216, Facult\u00e9 de Pharmacie de Paris, Universit\u00e9 Paris Descartes, Paris, France"},{"name":"UMR 216, Institut de Recherche pour le D\u00e9veloppement, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan I.","family":"Iorga","sequence":"additional","affiliation":[{"name":"Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, Labex LERMIT, Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Yi","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris-Sud, Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Fisher","sequence":"additional","affiliation":[{"name":"Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Meunier","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Universit\u00e9 Paris-Sud, Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb200105d"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1205651109"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005029"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2011.09.016"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1416611112"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04149-14"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.07.011"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm087"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02550-13"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/375599"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5029"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302195200"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp510022w"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-240"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2007.00328.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500388200"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2725-2731.2003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-028-7_5"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10465"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313866200"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.065"},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","first-page":"3977","DOI":"10.1074\/jbc.M606482200","article-title":"Mutational analysis of cytochrome b at the ubiquinol oxidation site of yeast complex III","volume":"282","author":"Wenz T","year":"2007","unstructured":"Wenz T, Covian R, Hellwig P, Macmillan F, Meunier B, Trumpower BL, Hunte C. 2007. Mutational analysis of cytochrome b at the ubiquinol oxidation site of yeast complex III. 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