{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:12:11Z","timestamp":1791414731158,"version":"4.3.3"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ivermectin and moxidectin are the most widely administered anthelmintic macrocyclic lactones (MLs) to treat human and animal nematode infections. Their widespread and frequent use has led to a high level of resistance to these drugs. Although they have the same mode of action, differences in terms of selection for drug resistance have been reported. Our objective was to study and compare changes occurring upon ivermectin or moxidectin selection in the model nematode\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Caenorhabditis elegans<\/jats:named-content>\n                    .\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. elegans<\/jats:named-content>\n                    worms were submitted to stepwise exposure to increasing doses of moxidectin. The sensitivity of moxidectin-selected worms to MLs was determined in a larval development assay and compared with those of wild-type and ivermectin-selected strains. Selection with either ivermectin or moxidectin led to acquired tolerance to ivermectin, moxidectin, and eprinomectin. Importantly, moxidectin was the most potent ML in both ivermectin- and moxidectin-selected strains. Interestingly, this order of potency was also observed in a resistant\n                    <jats:italic>Haemonchus contortus<\/jats:italic>\n                    isolate. In addition, ivermectin- and moxidectin-selected strains displayed constitutive overexpression of several genes involved in xenobiotic metabolism and transport. Moreover, verapamil potentiated sensitivity to ivermectin and moxidectin, demonstrating that ABC transporters play a role in ML sensitivity in ML-selected\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. elegans<\/jats:named-content>\n                    strains. Finally, both ivermectin- and moxidectin-selected strains displayed a dye-filling-defective phenotype. Overall, this work demonstrated that selection with ivermectin or moxidectin led to cross-resistance to several MLs in nematodes and that the induction of detoxification systems and defects in the integrity of amphidial neurons are two mechanisms that appear to affect the responsiveness of worms to both ivermectin and moxidectin.\n                  <\/jats:p>","DOI":"10.1128\/aac.00713-16","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T23:18:44Z","timestamp":1464736724000},"page":"4809-4819","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Acquired Tolerance to Ivermectin and Moxidectin after Drug Selection Pressure in the Nematode Caenorhabditis elegans"],"prefix":"10.1128","volume":"60","author":[{"given":"C\u00e9cile","family":"M\u00e9nez","sequence":"first","affiliation":[{"name":"Toxalim, Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Alberich","sequence":"additional","affiliation":[{"name":"Toxalim, Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"Kansoh","sequence":"additional","affiliation":[{"name":"Toxalim, Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Blanchard","sequence":"additional","affiliation":[{"name":"INRA, UMR 1282 Infectiology and Public Health, Nouzilly, France"},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Lespine","sequence":"additional","affiliation":[{"name":"Toxalim, Universit\u00e9 de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2005.08.014"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2012.04.001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002953"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007000066"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.08.001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60942-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.07.010"},{"key":"e_1_3_2_9_2","first-page":"290","article-title":"Effects of ivermectin and moxidectin on the transcription of genes coding for multidrug resistance associated proteins and behaviour in Caenorhabditis elegans","volume":"40","author":"Ardelli BF","year":"2008","unstructured":"Ardelli BF, Prichard R. 2008. 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