{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:49:31Z","timestamp":1790095771568,"version":"4.0.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Umea University"},{"DOI":"10.13039\/501100011777","name":"Scandinavian Society for Antimicrobial Chemotherapy Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011777","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Swedish Government Funds for Clinical Research"},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Molecular Infection Medicine Sweden"},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI100759"],"award-info":[{"award-number":["R01AI100759"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The type II fatty acid synthesis (FASII) pathway is essential for bacterial lipid biosynthesis and continues to be a promising target for novel antibacterial compounds. Recently, it has been demonstrated that\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            is capable of FASII and this pathway is indispensable for\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            growth. Previously, a high-content screen with\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            -infected cells was performed, and acylated sulfonamides were identified to be potent growth inhibitors of the bacteria.\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            strains resistant to acylated sulfonamides were isolated by serial passage of a wild-type strain in the presence of low compound concentrations. Results from whole-genome sequencing of 10 isolates from two independent drug-resistant populations revealed that mutations that accumulated in\n            <jats:italic>fabF<\/jats:italic>\n            were predominant. Studies of the interaction between the FabF protein and small molecules showed that acylated sulfonamides directly bind to recombinant FabF\n            <jats:italic>in vitro<\/jats:italic>\n            and treatment of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            -infected HeLa cells with the compounds leads to a decrease in the synthesis of\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            fatty acids. This work demonstrates the importance of FASII for\n            <jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>\n            development and may lead to the development of new antimicrobials.\n          <\/jats:p>","DOI":"10.1128\/aac.00716-17","type":"journal-article","created":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T00:40:19Z","timestamp":1502152819000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["N-Acylated Derivatives of Sulfamethoxazole Block Chlamydia Fatty Acid Synthesis and Interact with FabF"],"prefix":"10.1128","volume":"61","author":[{"given":"Sergio A.","family":"Mojica","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olli","family":"Salin","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Bastidas","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology and Center for the Genomics of Microbial Systems, Duke University Medical Center, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh","family":"Sunduru","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Hedenstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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