{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:30:18Z","timestamp":1790314218485,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100011858","name":"African Academy of Sciences","doi-asserted-by":"publisher","award":["107755\/Z\/15\/Z"],"award-info":[{"award-number":["107755\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/501100011858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MC_EX_MR\/K02440X"],"award-info":[{"award-number":["MC_EX_MR\/K02440X"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,12,16]]},"abstract":"<jats:p>\n            Monitoring of\n            <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n            sensitivity to antimalarial drugs in Africa is vital for malaria elimination. However, the commonly used\n            <jats:italic>ex vivo<\/jats:italic>\n            \/\n            <jats:italic>in vitro<\/jats:italic>\n            50% inhibitory concentration (IC\n            <jats:sub>50<\/jats:sub>\n            ) test gives inconsistent results for several antimalarials, while the alternative ring-stage survival assay (RSA) for artemisinin derivatives has not been widely adopted. Here, we applied an alternative two-color flow cytometry-based parasite survival rate assay (PSRA) to detect\n            <jats:italic>ex vivo<\/jats:italic>\n            antimalarial tolerance in\n            <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n            isolates from The Gambia.\n          <\/jats:p>","DOI":"10.1128\/aac.00720-20","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T14:37:56Z","timestamp":1601995076000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Tolerance of Gambian Plasmodium falciparum to Dihydroartemisinin and Lumefantrine Detected by\n            <i>Ex Vivo<\/i>\n            Parasite Survival Rate Assay"],"prefix":"10.1128","volume":"65","author":[{"given":"Haddijatou","family":"Mbye","sequence":"first","affiliation":[{"name":"West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Ghana"},{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatoumata","family":"Bojang","sequence":"additional","affiliation":[{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aminata Seedy","family":"Jawara","sequence":"additional","affiliation":[{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bekai","family":"Njie","sequence":"additional","affiliation":[{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuredin Ibrahim","family":"Mohammed","sequence":"additional","affiliation":[{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Okebe","sequence":"additional","affiliation":[{"name":"Department of International Public Health, Liverpool School of Tropical Medicine, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umberto","family":"D\u2019Alessandro","sequence":"additional","affiliation":[{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"},{"name":"London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4478-3601","authenticated-orcid":false,"given":"Alfred","family":"Amambua-Ngwa","sequence":"additional","affiliation":[{"name":"MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia"},{"name":"London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.30875\/50d27d62-en"},{"key":"e_1_3_3_3_2","volume-title":"Status report on artemisinin resistance and ACT efficacy (August 2018)","author":"World Health Organization","year":"2018","unstructured":"World Health Organization. 2018. Status report on artemisinin resistance and ACT efficacy (August 2018). World Health Organization, Geneva, Swithzerland."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808859"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0805011"},{"key":"e_1_3_3_6_2","volume-title":"Artemisinin and artemisinin-based combination therapy resistance: status report","author":"World Health Organization","year":"2017","unstructured":"World Health Organization. 2017. Artemisinin and artemisinin-based combination therapy resistance: status report. World Health Organization, Geneva, Swithzerland."},{"key":"e_1_3_3_7_2","volume-title":"Eliminating malaria: Global Malaria Programme","author":"World Health Organization","year":"2016","unstructured":"World Health Organization. 2016. Eliminating malaria: Global Malaria Programme. 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