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An international strain collection of 361\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n            isolates from 258 CF patients seen at 30 CF clinics was examined for mutations in 17 antimicrobial susceptibility and resistance loci that had been identified as hot spots of mutation by genome sequencing of serial isolates from a single CF clinic. Combinatorial amplicon sequencing of pooled PCR products identified 1,112 sequence variants that were not present in the genomes of representative strains of the 20 most common clones of the global\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n            population. A high frequency of singular coding variants was seen in\n            <jats:italic>spuE<\/jats:italic>\n            ,\n            <jats:italic>mexA<\/jats:italic>\n            ,\n            <jats:italic>gyrA<\/jats:italic>\n            ,\n            <jats:italic>rpoB<\/jats:italic>\n            ,\n            <jats:italic>fusA1<\/jats:italic>\n            ,\n            <jats:italic>mexZ<\/jats:italic>\n            ,\n            <jats:italic>mexY<\/jats:italic>\n            ,\n            <jats:italic>oprD<\/jats:italic>\n            ,\n            <jats:italic>ampD<\/jats:italic>\n            ,\n            <jats:italic>parR<\/jats:italic>\n            ,\n            <jats:italic>parS<\/jats:italic>\n            , and\n            <jats:italic>envZ<\/jats:italic>\n            (\n            <jats:italic>amgS<\/jats:italic>\n            ), reflecting the pressure upon\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n            in lungs of CF patients to generate novel protein variants. The proportion of nonneutral amino acid exchanges was high. Of the 17 loci,\n            <jats:italic>mexA<\/jats:italic>\n            ,\n            <jats:italic>mexZ<\/jats:italic>\n            , and\n            <jats:italic>pagL<\/jats:italic>\n            were most frequently affected by independent stop mutations. Private and\n            <jats:italic>de novo<\/jats:italic>\n            mutations seem to play a pivotal role in the response of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n            populations to the antimicrobial load and the individual CF host.\n          <\/jats:p>","DOI":"10.1128\/aac.00724-16","type":"journal-article","created":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T02:03:38Z","timestamp":1472522618000},"page":"6726-6734","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Molecular Epidemiology of Mutations in Antimicrobial Resistance Loci of Pseudomonas aeruginosa Isolates from Airways of Cystic Fibrosis Patients"],"prefix":"10.1128","volume":"60","author":[{"given":"Leonie","family":"Greipel","sequence":"first","affiliation":[{"name":"Clinical Research 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