{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:46:40Z","timestamp":1791049600852,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["NOA21NU51CK000316"],"award-info":[{"award-number":["NOA21NU51CK000316"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,11,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Since 2016,\n Candida auris<\/jats:italic>\n has been subject to mandatory reporting in Colombia due to its critical public health implications. In this context, a genomic characterization of clinical\n C. auris<\/jats:italic>\n isolates collected between 2018 and 2024 through national surveillance led by the Instituto Nacional de Salud (INS) was conducted. Twenty-seven isolates from hospitalized patients across 14 departments were analyzed using next-generation sequencing (NGS) and bioinformatics tools (MycoSNP-nf v1.5, TheiaEuk BLAST, Python script, and MUSCLE, snp_phylogeny) to identify mutations associated with antifungal resistance. The patient group was predominantly male\n n<\/jats:italic>\n = 18 (66.7%), with 48% (\n n<\/jats:italic>\n = 13) for the older adult age group (>60 years of age). Antifungal susceptibility testing (anidulafungin, fluconazole, and amphotericin B) revealed that five isolates (18.5%) were resistant to a single antifungal, while 22 (81.5%) were resistant to two. All isolates belonged to clade IV (South America) and exhibited genetic divergence ranging from 5 to 127 single-nucleotide polymorphisms (SNPs). Mutations in the\n ERG11<\/jats:italic>\n gene were identified in 16 isolates, including Y132F (\n n<\/jats:italic>\n = 11), G459S (\n n<\/jats:italic>\n = 1), K143R (\n n<\/jats:italic>\n = 2), and F444L (\n n<\/jats:italic>\n = 1), along with a novel mutation, G445D (\n n<\/jats:italic>\n = 1). Four of these 16 isolates also harbored mutations in the\n TAC1B<\/jats:italic>\n gene (P595H,\n n<\/jats:italic>\n = 3; S611P,\n n<\/jats:italic>\n = 1), and one isolate carried a single\n TAC1B<\/jats:italic>\n mutation (S195G). Additionally, three isolates presented the S639F mutation in the\n FKS1<\/jats:italic>\n gene. Notably, seven isolates showed phenotypic resistance without any known resistance mutations. These findings are concerning, as the antifungals involved are critical for treating invasive\n Candida<\/jats:italic>\n infections. The study highlights the need for ongoing genomic surveillance to complement phenotypic testing and enhance strategies for antifungal resistance control, ultimately informing therapeutic decision-making and drug development.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/aac.00725-25","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T13:17:53Z","timestamp":1759843073000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Genomic epidemiology of Colombian resistant\n Candida auris<\/i>\n isolates"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0738-0056","authenticated-orcid":true,"given":"Maira Lyseth","family":"Alvarado Casas","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gx6zj11","id-type":"ROR","asserted-by":"publisher"}],"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5694-5939","authenticated-orcid":true,"given":"Jeisson Alejandro","family":"Triana D\u00edaz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gx6zj11","id-type":"ROR","asserted-by":"publisher"}],"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8551-6281","authenticated-orcid":true,"given":"Efrain","family":"Montilla-Escudero","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gx6zj11","id-type":"ROR","asserted-by":"publisher"}],"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6326-2003","authenticated-orcid":true,"given":"Diego Andr\u00e9s","family":"Prada Cardozo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gx6zj11","id-type":"ROR","asserted-by":"publisher"}],"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3029-118X","authenticated-orcid":true,"given":"Patricia","family":"Escand\u00f3n Hern\u00e1ndez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gx6zj11","id-type":"ROR","asserted-by":"publisher"}],"name":"Grupo de Microbiolog\u00eda, Instituto Nacional de Salud","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13390"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof9090913"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2019.103243"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw691"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.7082"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx480"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00365-20"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00238-18"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03364-19"},{"key":"e_1_3_3_11_2","unstructured":"Centers for Disease Control and Prevention. 2024. Antifungal susceptibility testing and interpretation. Available from: https:\/\/www.cdc.gov\/candida-auris\/hcp\/laboratories\/antifungal-susceptibility-testing.html. Retrieved 10 Mar 2025."},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.11.024"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2016.07.003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2019.05.008"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy411"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1136217"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3727"},{"key":"e_1_3_3_18_2","volume-title":"CLSI Standard M27","author":"CLSI","year":"2017","unstructured":"CLSI. 2017. Reference method for broth dilution antifungal susceptibility testing of yeasts. In CLSI Standard M27, 4th ed. 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