{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T18:10:34Z","timestamp":1783361434493,"version":"3.54.6"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Veterans Health Administration","award":["I01 CX000920"],"award-info":[{"award-number":["I01 CX000920"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>H<\/jats:italic>\n            30 subclone of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            sequence type 131 (ST131-\n            <jats:italic>H<\/jats:italic>\n            30) has become the leading antimicrobial resistance\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            lineage in the United States and often exhibits resistance to one or both of the two key antimicrobial classes for treating Gram-negative infections, extended-spectrum cephalosporins (ESCs) and fluoroquinolones (FQs). However, the timing of and reasons for its recent emergence are inadequately defined. Accordingly, from\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            clinical isolates collected systematically across the United States by the SENTRY Antimicrobial Surveillance Program in 2000, 2003, 2006, and 2009, 234 isolates were selected randomly, stratified by year, within three resistance categories: (i) ESC-reduced susceptibility, regardless of FQ phenotype (ESC-RS); (ii) FQ resistance, ESC susceptible (FQ-R); and (iii) FQ susceptible, ESC susceptible (FQ-S). Susceptibility profiles, phylogroup, ST, ST131 subclone, and virulence genotypes were determined, and temporal trends and between-variable associations were assessed statistically. From 2000 to 2006, concurrently with the emergence of ESC-RS and FQ-R strains, the prevalence of (virulence-associated) phylogroup B2 among such strains also rose dramatically, due entirely to rapid emergence of ST131, especially\n            <jats:italic>H<\/jats:italic>\n            30. By 2009,\n            <jats:italic>H<\/jats:italic>\n            30 was the dominant\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            lineage overall (22%), accounting for a median of 43% of all single-agent and multidrug resistance (68% for ciprofloxacin).\n            <jats:italic>H<\/jats:italic>\n            30's emergence increased the net virulence gene content of resistant (especially FQ-R) isolates, giving stable overall virulence gene scores despite an approximately 4-fold expansion of the historically less virulent resistant population. These findings define more precisely the timing and tempo of\n            <jats:italic>H<\/jats:italic>\n            30's emergence in the United States, identify possible reasons for it, and suggest potential consequences, including more frequent and\/or aggressive antimicrobial-resistant infections.\n          <\/jats:p>","DOI":"10.1128\/aac.00732-17","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:31:13Z","timestamp":1495499473000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Epidemic Emergence in the United States of Escherichia coli Sequence Type 131-\n            <i>H<\/i>\n            30 (ST131-\n            <i>H<\/i>\n            30), 2000 to 2009"],"prefix":"10.1128","volume":"61","author":[{"given":"James R.","family":"Johnson","sequence":"first","affiliation":[{"name":"Minneapolis VA Medical Center, Minneapolis, Minnesota, USA"},{"name":"University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Porter","sequence":"additional","affiliation":[{"name":"Minneapolis VA Medical Center, Minneapolis, Minnesota, USA"},{"name":"University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Thuras","sequence":"additional","affiliation":[{"name":"Minneapolis VA Medical Center, Minneapolis, Minnesota, USA"},{"name":"University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Castanheira","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(03)00049-2"},{"key":"e_1_3_2_3_2","first-page":"33","volume-title":"Annual epidemiological commentary: mandatory MRSA, MSSA and E. coli bacteraemia and C. difficile infection data 2015\/16","author":"Thelwall S","year":"2016","unstructured":"Thelwall S NsonwuO WastiS ElmiM GerverS DaviesJ HopeR. 2016. Annual epidemiological commentary: mandatory MRSA, MSSA and E. coli bacteraemia and C. difficile infection data 2015\/16, p 33\u201342. 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