{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T05:04:14Z","timestamp":1791003854011,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            carbapenemase (KPC) variant, designated\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-5<\/jats:sub>\n            , was discovered in a carbapenem-resistant\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            clinical isolate from Puerto Rico. Characterization of the upstream region of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-5<\/jats:sub>\n            showed significant differences from the flanking regions of other\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            variants. Comparison of amino acid sequences with those of other KPC enzymes revealed that KPC-5 was an intermediate between KPC-2 and KPC-4, differing from KPC-2 by a single amino acid substitution (Pro\n            <jats:sub>103<\/jats:sub>\n            \u2192Arg), while KPC-4 contained Pro\n            <jats:sub>103<\/jats:sub>\n            \u2192Arg plus an additional amino acid change (Val\n            <jats:sub>239<\/jats:sub>\n            \u2192Gly). Transformation studies with an\n            <jats:italic>Escherichia coli<\/jats:italic>\n            recipient strain showed differences in the properties of the KPC variants. KPC-4 and KPC-5 both had pIs of 7.65, in contrast with the pI of 6.7 for KPC-2. KPC-2 transformants were less susceptible to the carbapenems than KPC-4 and KPC-5 transformants. These data correlated with higher rates of imipenem hydrolysis for KPC-2 than for KPC-4 and KPC-5. However, KPC-4 and KPC-5 transformants had higher ceftazidime MICs, and the enzymes from these transformants had slightly better hydrolysis of this drug than KPC-2. KPC-4 and KPC-5 were more sensitive than KPC-2 to inhibition by clavulanic acid in both susceptibility testing and hydrolysis assays. Thus, KPC enzymes may be evolving through stepwise mutations to alter their spectra of activity.\n          <\/jats:p>","DOI":"10.1128\/aac.00734-08","type":"journal-article","created":{"date-parts":[[2008,11,18]],"date-time":"2008-11-18T02:26:03Z","timestamp":1226975163000},"page":"557-562","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":100,"title":["Phenotypic and Enzymatic Comparative Analysis of the Novel KPC Variant KPC-5 and Its Evolutionary Variants, KPC-2 and KPC-4"],"prefix":"10.1128","volume":"53","author":[{"given":"Daniel J.","family":"Wolter","sequence":"first","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip M.","family":"Kurpiel","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Woodford","sequence":"additional","affiliation":[{"name":"Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London, NW9 5EQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-France I.","family":"Palepou","sequence":"additional","affiliation":[{"name":"Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London, NW9 5EQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard V.","family":"Goering","sequence":"additional","affiliation":[{"name":"Creighton University School of Medicine, Omaha, Nebraska"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy D.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4760-4762.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/421495"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.3018-3020.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01114-07"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4745-4750.2005"},{"key":"e_1_3_2_8_2","unstructured":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 2008 vol. 28"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.07.004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2652-2658.2004"},{"key":"e_1_3_2_11_2","first-page":"455","volume":"61","year":"2008","unstructured":"Dortet, L., I. 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