{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:55:45Z","timestamp":1785138945612,"version":"3.55.0"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Genetic characterization of methicillin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus pseudintermedius<\/jats:named-content>\n            (MRSP) from Thailand and Israel revealed the presence of a predominant atypical clonal lineage which was not typeable by SmaI-PFGE and SCC\n            <jats:italic>mec<\/jats:italic>\n            typing. All the atypical isolates (\n            <jats:italic>n<\/jats:italic>\n            = 34) belonged to CC45 (30 ST45 and 2 ST179 isolates, 1 ST57 isolate, and 1 ST85 isolate). The isolates originated from healthy and diseased dogs and cats, as well as from the environment of one clinic. Cfr9I\u2013pulsed-field gel electrophoresis (Cfr9I-PFGE) and\n            <jats:italic>dru<\/jats:italic>\n            typing permitted the further distinction of CC45 isolates from the two different countries. Microarray analysis identified genes that confer resistance to \u03b2-lactams (\n            <jats:italic>mecA<\/jats:italic>\n            ;\n            <jats:italic>blaZ<\/jats:italic>\n            ), aminoglycosides [\n            <jats:italic>aac(6\u2032)-Ie\u2013aph(2\u2032)-Ia<\/jats:italic>\n            ;\n            <jats:italic>aph(3\u2032)-III<\/jats:italic>\n            ;\n            <jats:italic>ant(6)-Ia<\/jats:italic>\n            ], macrolides and lincosamides [\n            <jats:italic>erm<\/jats:italic>\n            (B)], tetracyclines [\n            <jats:italic>tet<\/jats:italic>\n            (M)], trimethoprim [\n            <jats:italic>dfr<\/jats:italic>\n            (G)], streptothricin (\n            <jats:italic>sat4<\/jats:italic>\n            ), and chloramphenicol (\n            <jats:italic>cat<\/jats:italic>\n            <jats:sub>pC221<\/jats:sub>\n            ). Fluoroquinolone resistance was attributed to specific amino acid substitutions, i.e., Ser84Leu in GyrA and Ser80Ile and Asp84Asn in GrlA. A novel pseudo-staphylococcal cassette chromosome (\u03a8SCC\n            <jats:italic>mec<\/jats:italic>\n            <jats:sub>57395<\/jats:sub>\n            ) element was identified in MRSP strain 57395 (sequence type ST45) by whole-genome sequencing. The 12,282-bp \u03a8SCC\n            <jats:italic>mec<\/jats:italic>\n            <jats:sub>57395<\/jats:sub>\n            element contained a class C1\n            <jats:italic>mec<\/jats:italic>\n            gene complex but no\n            <jats:italic>ccr<\/jats:italic>\n            genes. In addition to the methicillin resistance gene\n            <jats:italic>mecA<\/jats:italic>\n            , \u03a8SCC\n            <jats:italic>mec<\/jats:italic>\n            <jats:sub>57395<\/jats:sub>\n            also carried determinants of resistance to heavy metals, such as arsenic, cadmium, and copper. Bsu36I restriction analysis of the \u03a8SCC\n            <jats:italic>mec<\/jats:italic>\n            <jats:sub>57395<\/jats:sub>\n            element amplified by long-range PCR revealed the presence of \u03a8SCC\n            <jats:italic>mec<\/jats:italic>\n            <jats:sub>57395<\/jats:sub>\n            in the 33 additional isolates of MRSP CC45. The \u03a8SCC\n            <jats:italic>mec<\/jats:italic>\n            <jats:sub>57395<\/jats:sub>\n            element represents a new class of SCC\n            <jats:italic>mec<\/jats:italic>\n            and has been identified in MRSP of CC45, which is a predominant clonal lineage in Israel and Thailand.\n          <\/jats:p>","DOI":"10.1128\/aac.00738-13","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T04:14:23Z","timestamp":1377576863000},"page":"5509-5515","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Novel Pseudo-Staphylococcal Cassette Chromosome\n            <i>mec<\/i>\n            Element (\u03a8SCC\n            <i>mec<\/i>\n            <sub>57395<\/sub>\n            ) in Methicillin-Resistant Staphylococcus pseudintermedius CC45"],"prefix":"10.1128","volume":"57","author":[{"given":"Vincent","family":"Perreten","sequence":"first","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pattrarat","family":"Chanchaithong","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"},{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuvee","family":"Prapasarakul","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Rossano","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shlomo E.","family":"Blum","sequence":"additional","affiliation":[{"name":"Division of Bacteriology and Mycology, The Kimron Veterinary Institute, Bet Dagan, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Elad","sequence":"additional","affiliation":[{"name":"Division of Bacteriology and Mycology, The Kimron Veterinary Institute, Bet Dagan, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sybille","family":"Schwendener","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, 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