{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:12:55Z","timestamp":1785420775422,"version":"3.56.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2307103"],"award-info":[{"award-number":["2023YFC2307103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402672"],"award-info":[{"award-number":["82402672"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81991531"],"award-info":[{"award-number":["81991531"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      A novel fosfomycin-resistant glutathione S-transferase (FR-GST) gene,\n                      <jats:italic toggle=\"yes\">fosZ<\/jats:italic>\n                      , was investigated, and its structural characteristics were characterized in\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      species. The\n                      <jats:italic toggle=\"yes\">fosZ<\/jats:italic>\n                      gene was cloned and expressed in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      PAO1 and HS355, where it displayed reduced susceptibility to fosfomycin (8- to 64-fold) and its inhibitor sodium phosphonoformate (PPF). FosZ shares less than 70% amino acid identity with known FosA proteins. Bioinformatics analyzes revealed that\n                      <jats:italic toggle=\"yes\">fosZ<\/jats:italic>\n                      was a transposable passenger gene within IS\n                      <jats:italic toggle=\"yes\">Pa75<\/jats:italic>\n                      , likely captured from\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      species. A total of 159\n                      <jats:italic toggle=\"yes\">fosZ<\/jats:italic>\n                      -bearing\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      strains were identified in GenBank over the past 22 years, sharing ten target site duplications (TSDs) associated with IS\n                      <jats:italic toggle=\"yes\">Pa75<\/jats:italic>\n                      . Among them, 34 strains were fully sequenced. IS\n                      <jats:italic toggle=\"yes\">Pa75-fosZ<\/jats:italic>\n                      was found on at least two chromosomes and 33 plasmids from four incompatibility groups, including the megaplasmids IncP-2 (24\/33) and Inc\n                      <jats:sub>pJBCL41<\/jats:sub>\n                      (8\/33), which also carried carbapenem and other antibiotic resistance genes. The most common TSDs were TSD6 (82\/156) and TSD10 (48\/156), predominantly in carbapenem-resistant\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      of various sequence types, especially ST1076, ST1418, and ST463. Mobilization of IS\n                      <jats:italic toggle=\"yes\">Pa75<\/jats:italic>\n                      between plasmid and chromosome was observed in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      MAS152. Structure prediction and analysis of FR-GST proteins revealed distinct features in the dimer interface loop. In the FosZ structure, an expanded K\n                      <jats:sup>+<\/jats:sup>\n                      -binding loop caused a deviation at residue S95, which participated in binding both fosfomycin and PPF. In conclusion,\n                      <jats:italic toggle=\"yes\">fosZ<\/jats:italic>\n                      encodes a distinct FR-GST exhibiting reduced susceptibility to inhibition by PPF and has been acquired by transferable IS\n                      <jats:italic toggle=\"yes\">Pa75<\/jats:italic>\n                      on multidrug-resistant megaplasmids, disseminating fosfomycin resistance in\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      species, particularly across China.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00750-25","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:00:23Z","timestamp":1768399223000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["<i>fosZ<\/i>\n                    , a novel plasmid-borne fosfomycin resistance gene in\n                    <i>Pseudomonas<\/i>\n                    species, especially carbapenem-resistant\n                    <i>Pseudomonas aeruginosa<\/i>\n                    isolates"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0099-764X","authenticated-orcid":false,"given":"Youxing","family":"Shao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013q1eq08","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Antibiotics, Huashan Hospital, Fudan University","place":["Shanghai, People's Republic of China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Lan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013q1eq08","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Antibiotics, Huashan Hospital, Fudan University","place":["Shanghai, People's Republic of China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4262-5290","authenticated-orcid":false,"given":"Xuefei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leilei","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013q1eq08","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Antibiotics, Huashan Hospital, Fudan University","place":["Shanghai, People's Republic of China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9604-913X","authenticated-orcid":true,"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013q1eq08","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Antibiotics, Huashan Hospital, Fudan University","place":["Shanghai, People's Republic of China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7682-5859","authenticated-orcid":true,"given":"Minggui","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013q1eq08","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Antibiotics, Huashan Hospital, Fudan University","place":["Shanghai, People's Republic of China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2490-1201","authenticated-orcid":true,"given":"Qinglan","family":"Guo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013q1eq08","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Antibiotics, Huashan Hospital, Fudan University","place":["Shanghai, People's Republic of China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00031-19"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00068-15"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz149"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa095"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01446-21"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00749-17"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01834-09"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw177"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00410-17"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01403-19"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S251360"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02542-23"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03131-14"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02042-19"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01424-17"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01094-14"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2021.1894903"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab342"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2024.107119"},{"key":"e_1_3_4_21_2","series-title":"Supplement M102","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute","year":"2024","unstructured":"Clinical and Laboratory Standards Institute. 2024. Performance standards for antimicrobial susceptibility testing. 34rd ed. Supplement M102. The Institute, Wayne, PA.","edition":"34"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad359"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2058421"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01572-17"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048767h"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2011.06.006"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2009.02.003"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04986-22"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00213"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02035-22"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab044"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00135-23"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.cn112147-20220407-00290"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2023.01.017"},{"key":"e_1_3_4_37_2","unstructured":"European Committee on Antimicrobial Susceptibility Testing. 2022. Breakpoint tables for interpretation of MICs and zone diameters. Available from: https:\/\/www.eucast.org\/ast_of_bacteria\/disk_diffusion_methodology"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00750-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00750-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:01:34Z","timestamp":1770213694000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00750-25"}},"subtitle":[],"editor":[{"given":"Alessandra","family":"Carattoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,2,4]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2,4]]}},"alternative-id":["10.1128\/aac.00750-25"],"URL":"https:\/\/doi.org\/10.1128\/aac.00750-25","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,4]]},"assertion":[{"value":"2025-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00750-25"}}