{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:34:53Z","timestamp":1791264893927,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum \u03b2-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two\n Escherichia coli<\/jats:named-content>\n and three\n Klebsiella pneumoniae<\/jats:named-content>\n subsp.\n pneumoniae<\/jats:named-content>\n isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids.\n E. coli<\/jats:named-content>\n isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n and\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n , while\n K. pneumoniae<\/jats:named-content>\n subsp.\n pneumoniae<\/jats:named-content>\n isolates carried\n bla<\/jats:italic>\n SHV-12<\/jats:sub>\n and\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n . Conjugation experiments demonstrated that\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n and\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n were carried on a conjugative plasmid in\n E. coli<\/jats:named-content>\n , and PBRT identified this to be an IncI1 plasmid. The resistance genes were nontransferable in\n K. pneumoniae<\/jats:named-content>\n subsp.\n pneumoniae<\/jats:named-content>\n isolates. Moreover, in the\n E. coli<\/jats:named-content>\n isolates, an association of IS\n Ecp<\/jats:italic>\n 1 and IS\n 26<\/jats:italic>\n with\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n was found where the IS\n 26<\/jats:italic>\n element was inserted upstream of both IS\n Ecp1<\/jats:italic>\n and the\n bla<\/jats:italic>\n CTX-M<\/jats:sub>\n promoter, a genetic arrangement highly similar to that described in some United Kingdom human isolates. We report the first cases in Europe of bovine mastitis due to\n E. coli<\/jats:named-content>\n CTX-M-15 and also of bovine mastitis due to\n K. pneumoniae<\/jats:named-content>\n subsp.\n pneumoniae<\/jats:named-content>\n SHV-12 \u03b2-lactamases in the United Kingdom. We also describe the genetic environment of\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n and highlight the role that IncI1 plasmids may play in the spread and dissemination of ESBL genes, which have been described in both human and cattle isolates.\n <\/jats:p>","DOI":"10.1128\/aac.00752-13","type":"journal-article","created":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T23:59:32Z","timestamp":1384819172000},"page":"789-794","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":94,"title":["Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 \u03b2-Lactamases from Bovine Mastitis Isolates in the United Kingdom"],"prefix":"10.1128","volume":"58","author":[{"given":"Dorina","family":"Timofte","sequence":"first","affiliation":[{"name":"School of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom"},{"name":"Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iuliana E.","family":"Maciuca","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Evans","sequence":"additional","affiliation":[{"name":"Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Williams","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Wattret","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny C.","family":"Fick","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, University of Liverpool, Leahurst, Cheshire, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola J.","family":"Williams","sequence":"additional","affiliation":[{"name":"Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.160.8.253"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2005.06.002"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp433"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-8-21"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02831-10"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq396"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-009-9263-y"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00941-10"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.10.015"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00920-13"},{"key":"e_1_3_1_12_2","volume-title":"CLSI document M31-A3.","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. 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