{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:06:24Z","timestamp":1753880784731,"version":"3.41.2"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Tiroler Wissenschaftsfonds TWF","award":["2012-1-19"],"award-info":[{"award-number":["2012-1-19"]}]},{"DOI":"10.13039\/501100009980","name":"Medical University of Innsbruck","doi-asserted-by":"crossref","award":["MUI start grant 19970"],"award-info":[{"award-number":["MUI start grant 19970"]}],"id":[{"id":"10.13039\/501100009980","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["I 661"],"award-info":[{"award-number":["I 661"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Morphological heterogeneity of\n            <jats:named-content content-type=\"genus-species\">Aspergillus terreus<\/jats:named-content>\n            cultures was observed during continued cultivation of amphotericin B (AMB)-resistant isolates on drug-free medium. Outgrowth leads to the emergence of multiple sectors that might result from increased growth rates at drug-free conditions. We evaluated the differences in AMB susceptibility and virulence between sector subcultures (ATSec), AMB-resistant (ATR) strains, and AMB-susceptible (ATS) strains. By comparing\n            <jats:named-content content-type=\"genus-species\">A. terreus<\/jats:named-content>\n            AMB-resistant (ATR) strains and\n            <jats:named-content content-type=\"genus-species\">A. terreus<\/jats:named-content>\n            sector (ATSec) cultures we observed a highly significant reduction of AMB MICs in ATSec (ATR MIC, 2 to 32 \u03bcg\/ml; ATSec MIC, 0.12 to 5 \u03bcg\/ml). Furthermore,\n            <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n            survival studies revealed an enhanced virulence of ATSec, which was comparable with that of AMB-sensitive\n            <jats:named-content content-type=\"genus-species\">Aspergillus terreus<\/jats:named-content>\n            strains (median survival rates for ATS isolates, 72 h; for ATSec isolate ATSec\n            <jats:sup>G1<\/jats:sup>\n            , 84 h; for ATR isolates, 144 h). Our findings clearly demonstrate that spontaneous culture degeneration occurs in\n            <jats:named-content content-type=\"genus-species\">A. terreus<\/jats:named-content>\n            and, most importantly, crucially impacts drug efficacy and virulence.\n          <\/jats:p>","DOI":"10.1128\/aac.00755-17","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T00:50:18Z","timestamp":1507596618000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Impact of Morphological Sectors on Antifungal Susceptibility Testing and Virulence Studies"],"prefix":"10.1128","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6909-7005","authenticated-orcid":false,"given":"Emina","family":"Jukic","sequence":"first","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"},{"name":"Division of Human Genetics, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Blatzer","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Binder","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Mayr","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Lass-Fl\u00f6rl","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Lackner","sequence":"additional","affiliation":[{"name":"Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2307\/3761688"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.028480"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01682.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30138-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01024"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.01.009"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov025"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.09.003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2000644"},{"key":"e_1_3_3_11_2","unstructured":"Arendrup\nMC GuineaJ Cuenca-EstrellaM MeletiadisJ MoutonJW LagrouK HowardSJ Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST). 2015. EUCAST Definitive Document E.DEF 9.3. Method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming moulds. EUCAST antifungal MIC method for moulds. http:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/AFST\/Files\/EUCAST_E_Def_9_3_Mould_testing_definitive.pdf."},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2009.09.002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.6220"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1045183"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-12-14-0411-R"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00572660"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/014332-0"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01283-12"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2010.12.011"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05164-14"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00755-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00755-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:46:34Z","timestamp":1645476394000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00755-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":19,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1128\/AAC.00755-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.00755-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"type":"print","value":"0066-4804"},{"type":"electronic","value":"1098-6596"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"2017-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00755-17"}}