{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:52:44Z","timestamp":1790175164237,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The yeast\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    is an opportunistic human fungal pathogen and the cause of superficial and systemic infections in immunocompromised patients. The classes of antifungal agents most commonly used to treat\n                    <jats:italic>Candida<\/jats:italic>\n                    infections are the azoles, polyenes, and echinocandins. In the present study, we identified changes in\n                    <jats:italic>C. albicans<\/jats:italic>\n                    protein abundance using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectroscopy following exposure to representatives of the azole (ketoconazole), polyene (amphotericin B), and echinocandin (caspofungin) antifungals in an effort to elucidate the adaptive responses to these classes of antifungal agents. We identified 39 proteins whose abundance changed in response to ketoconazole exposure. Some of these proteins are involved in ergosterol biosynthesis and are associated with azole resistance. Exposure to amphotericin B altered the abundance of 43 proteins, including those associated with oxidative stress and osmotic tolerance. We identified 50 proteins whose abundance changed after exposure to caspofungin, including enzymes involved in cell wall biosynthesis and integrity, as well as the regulator of \u03b2-1,3-glucan synthase activity, Rho1p. Exposure to caspofungin also increased the abundance of the proteins involved in oxidative and osmotic stress. The common adaptive responses shared by all three antifungal agents included proteins involved in carbohydrate metabolism. Some of these antifungal-responsive proteins may represent potential targets for the development of novel therapeutics that could enhance the antifungal activities of these drugs.\n                  <\/jats:p>","DOI":"10.1128\/aac.00756-09","type":"journal-article","created":{"date-parts":[[2010,2,9]],"date-time":"2010-02-09T23:54:38Z","timestamp":1265759678000},"page":"1655-1664","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Changes in the Proteome of\n                    <i>Candida albicans<\/i>\n                    in Response to Azole, Polyene, and Echinocandin Antifungal Agents"],"prefix":"10.1128","volume":"54","author":[{"given":"Christopher F.","family":"Hoehamer","sequence":"first","affiliation":[{"name":"Departments of Clinical Pharmacy, Pharmaceutical Sciences, and Pediatrics, Colleges of Pharmacy and Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163"},{"name":"Children's Foundation Research Center of Memphis, Le Bonheur Children's Medical Center, Memphis, Tennessee 38103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin D.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George M.","family":"Hilliard","sequence":"additional","affiliation":[{"name":"Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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