{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:10:24Z","timestamp":1767625824293},"reference-count":77,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            L-forms are cell wall-deficient bacteria that can grow and proliferate in osmotically stabilizing media. Recently, a strain of the Gram-positive model bacterium\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Bacillus subtilis<\/jats:named-content>\n            was constructed that allowed controlled switching between rod-shaped wild-type cells and corresponding L-forms. Both states can be stably maintained under suitable culture conditions. Because of the absence of a cell wall, L-forms are known to be insensitive to \u03b2-lactam antibiotics, but reports on the susceptibility of L-forms to other antibiotics that interfere with membrane-anchored steps of cell wall biosynthesis are sparse, conflicting, and strongly influenced by strain background and method of L-form generation. Here we investigated the response of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. subtilis<\/jats:named-content>\n            to the presence of cell envelope antibiotics, with regard to both antibiotic resistance and the induction of the known LiaRS- and BceRS-dependent cell envelope stress biosensors. Our results show that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. subtilis<\/jats:named-content>\n            L-forms are resistant to antibiotics that interfere with the bactoprenol cycle, such as bacitracin, vancomycin, and mersacidin, but are hypersensitive to nisin and daptomycin, which both affect membrane integrity. Moreover, we established a\n            <jats:italic>lacZ<\/jats:italic>\n            -based reporter gene assay for L-forms and provide evidence that LiaRS senses its inducers indirectly (damage sensing), while the Bce module detects its inducers directly (drug sensing).\n          <\/jats:p>","DOI":"10.1128\/aac.00770-12","type":"journal-article","created":{"date-parts":[[2012,9,11]],"date-time":"2012-09-11T06:10:15Z","timestamp":1347343815000},"page":"5907-5915","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Cell Envelope Stress Response in Cell Wall-Deficient L-Forms of Bacillus subtilis"],"prefix":"10.1128","volume":"56","author":[{"given":"Diana","family":"Wolf","sequence":"first","affiliation":[{"name":"Department Biology I, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Dom\u00ednguez-Cuevas","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Daniel","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorsten","family":"Mascher","sequence":"additional","affiliation":[{"name":"Department Biology I, Ludwig-Maximilians-University Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/j.1365-2672.1991.tb02946.x","article-title":"Induction and cultivation of a stable L form of Bacillus subtilis","volume":"70","author":"Allan EJ","year":"1991","unstructured":"AllanEJ. 1991. Induction and cultivation of a stable L form of Bacillus subtilis. J. Appl. Bacteriol. 70:339\u2013343.","journal-title":"J. Appl. Bacteriol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01132-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920109787048616"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314165200"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi970008u"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5448.2361"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.mimet.2008.05.002","article-title":"Microtiter plate bioassay to monitor the interference of antibiotics with the lipid II cycle essential for peptidoglycan biosynthesis","volume":"75","author":"Burkard M","year":"2008","unstructured":"BurkardM SteinT. 2008. 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