{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:02:41Z","timestamp":1791237761357,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Escherichia coli<\/jats:italic>\n            isolates recovered from patients during a clonal outbreak in a Warsaw, Poland, hospital in 1997 produced different levels of an extended-spectrum \u03b2-lactamase (ESBL) of the SHV type. The \u03b2-lactamase hyperproduction correlated with the multiplication of ESBL gene copies within a plasmid. Here, we present the complete nucleotide sequence of plasmid p1658\/97 carried by the isolates recovered during the outbreak. The plasmid is 125,491 bp and shows a mosaic structure in which all modules constituting the plasmid core are homologous to those found in plasmids F and R100 and are separated by segments of homology to other known regions (plasmid R64,\n            <jats:italic>Providencia rettgeri<\/jats:italic>\n            genomic island R391,\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            STX transposon,\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            or\n            <jats:italic>E. coli<\/jats:italic>\n            chromosomes). Plasmid p1658\/97 bears two replication systems, IncFII and IncFIB; we demonstrated that both are active in\n            <jats:italic>E. coli<\/jats:italic>\n            . The presence of an active partition system (\n            <jats:italic>sopABC<\/jats:italic>\n            locus) and two postsegregational killing systems (\n            <jats:italic>pemIK<\/jats:italic>\n            and\n            <jats:italic>hok\/sok<\/jats:italic>\n            ) indicates that the plasmid should be stably maintained in\n            <jats:italic>E. coli<\/jats:italic>\n            populations. The conjugative transfer is ensured by the operons of the\n            <jats:italic>tra<\/jats:italic>\n            and\n            <jats:italic>trb<\/jats:italic>\n            genes. We also demonstrate that the plasmidic segment undergoing amplification contains the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-5<\/jats:sub>\n            gene and is homologous to a 7.9-kb fragment of the\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            chromosome. The amplicon displays the structure of a composite transposon of type I.\n          <\/jats:p>","DOI":"10.1128\/aac.00772-06","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T01:49:47Z","timestamp":1168652987000},"page":"1164-1171","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Mosaic Structure of p1658\/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum \u03b2-Lactamase Gene\n            <i>bla<\/i>\n            <sub>SHV-5<\/sub>"],"prefix":"10.1128","volume":"51","author":[{"given":"M.","family":"Zienkiewicz","sequence":"first","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Kern-Zdanowicz","sequence":"additional","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Go\u0142e\u0327biewski","sequence":"additional","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Z\u0307ylin\u0303ska","sequence":"additional","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Mieczkowski","sequence":"additional","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Gniadkowski","sequence":"additional","affiliation":[{"name":"National Institute of Public Health, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bardowski","sequence":"additional","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Ceg\u0142owski","sequence":"additional","affiliation":[{"name":"Department of Microbial Biochemistry, Institute of Biochemistry, and Biophysics of Polish Academy of Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.6.1453-1461.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5331.1453"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.134.3.1141-1156.1978"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2856-2861.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh251"},{"key":"e_1_3_2_9_2","first-page":"1091","volume":"131","year":"1985","unstructured":"Fort, P., and J. Errington. 1985. Nucleotide sequence and complementation analysis of a polycistronic sporulation operon, spoVA, in Bacillus subtilis. J. Gen. Microbiol.131:1091-1105.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.5.685"},{"key":"e_1_3_2_11_2","first-page":"162","volume":"58","year":"1994","unstructured":"Frost, L. S., K. Ippen-Ihler, and R. A. Skurray. 1994. Analysis of the sequence and gene products of the transfer region of the F sex factor Microbiol. Rev.58:162-210.","journal-title":"Rev."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01975.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1198-743x.2001.00330.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.12.2705"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.256-263.2005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.165.3.1043-1045.1986"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.794-797.2005"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.2.261"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.725-774.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3541-3543.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.1.395-397.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00425660"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1151-1158.2004"},{"key":"e_1_3_2_25_2","unstructured":"Nielsen H. 1999. From sequence to sorting: prediction of signal peptides. Ph.D. thesis. Stockholm University Stockholm Sweden."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.393"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.657-686.2005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.164.3.1359-1361.1985"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.161.3.1042-1048.1985"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2003.09.005"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-619X(02)00017-3"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1999.1418"},{"key":"e_1_3_2_33_2","first-page":"2","volume":"6","year":"2000","unstructured":"Rahal, J. J. 2000. Extended-spectrum \u03b2-lactamases: how big is the problem? Clin. Microbiol. Infect.6:2-6.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.31.1.91"},{"key":"e_1_3_2_35_2","first-page":"2256","year":"1996","unstructured":"Roth, J. R., N. Benson, T. Galitski, K. Haack, J. G. Lawrence, and L. Meisel. 1996. Rearrangements of the bacterial chromosome: formation and applications, p. 2256-2276. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella. Cell and molecular biology. ASM Press, Washington, DC.","journal-title":"Escherichia coli and Salmonella"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.2.544"},{"key":"e_1_3_2_37_2","unstructured":"Sambrook E. F. J. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. 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