{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:28:18Z","timestamp":1790533698685,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A subset of bacterial pathogens, including the zoonotic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>\n            species, are highly resistant against polymyxin antibiotics. Bacterial polymyxin resistance has been attributed primarily to the modification of lipopolysaccharide; however, it is unknown what additional mechanisms mediate high-level resistance against this class of drugs. This work identified a role for the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella melitensis<\/jats:named-content>\n            gene\n            <jats:italic>bveA<\/jats:italic>\n            (\n            <jats:italic>BMEII0681<\/jats:italic>\n            ), encoding a predicted esterase, in the resistance of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>\n            to polymyxin B. Characterization of the enzymatic activity of BveA demonstrated that it is a phospholipase A1 with specificity for phosphatidylethanolamine (PE). Further, lipidomic analysis of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>\n            revealed an excess of PE lipids in the bacterial membranes isolated from the\n            <jats:italic>bveA<\/jats:italic>\n            mutant. These results suggest that by lowering the PE content of the cell envelope, BveA increases the resistance of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>\n            to polymyxin B. BveA was required for survival and replication of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>\n            in macrophages and for persistent infection in mice. BveA family esterases are encoded in the genomes of the alphaproteobacterial species that coexist with the polymyxin-producing bacteria in the rhizosphere, suggesting that maintenance of a low PE content in the bacterial cell envelope may be a shared persistence strategy for association with plant and mammalian hosts.\n          <\/jats:p>","DOI":"10.1128\/aac.00792-15","type":"journal-article","created":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T02:29:28Z","timestamp":1439864968000},"page":"6717-6724","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Phospholipase A1 Modulates the Cell Envelope Phospholipid Content of Brucella melitensis, Contributing to Polymyxin Resistance and Pathogenicity"],"prefix":"10.1128","volume":"59","author":[{"given":"Tobias","family":"Kerrinnes","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Briana M.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Leon","sequence":"additional","affiliation":[{"name":"West Coast Metabolomics Center, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle M.","family":"Roux","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya L.","family":"Atluri","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Winter","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.003451"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2007.12.003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm357"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-08"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00627a019"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/S0034-5288(18)33726-3","article-title":"The development of a new selective medium for the isolation of Brucella abortus from contaminated sources","volume":"16","author":"Farrell ID","year":"1974","unstructured":"Farrell ID. 1974. 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