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The parasites survive within mammalian macrophages and express a unique set of enzymes, the tryparedoxin peroxidases, for their defense against oxidative stress generated by the host. In this study, we demonstrate different roles of two distinct enzymes, the mitochondrial tryparedoxin peroxidase (mTXNPx) and the cytosolic tryparedoxin peroxidase (cTXNPx), in defending the parasites against mitochondrial and exogenous oxidative stress during infection and drug treatment. Our findings indicate a greater increase in cTXNPx expression in response to exogenous oxidative stress and a higher elevation of mTXNPx expression in response to mitochondrial or endogenous stress created by respiratory chain complex inhibitors. Overexpression of cTXNPx in\n                    <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n                    showed improved protection against exogenous stress and enhanced protection against mitochondrial stress in parasites overexpressing mTXNPx. Further, parasites overexpressing cTXNPx infected host cells with increased efficiency at early times of infection compared to control parasites or parasites overexpressing mTXNPx. The mTXNPx-overexpressing parasites maintained higher infection at later times. Higher mTXNPx expression occurred in wild-type parasites on exposure to miltefosine, while treatment with antimony elevated cTXNPx expression. Parasites resistant to miltefosine or antimony demonstrated increased expression of mTXNPx, as well as cTXNPx. In summary, this study provides evidence of distinct roles of the two enzymes defined by virtue of their localization during infection and drug treatment.\n                  <\/jats:p>","DOI":"10.1128\/aac.00806-17","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T20:40:25Z","timestamp":1508791225000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Functional Involvement of Leishmania donovani Tryparedoxin Peroxidases during Infection and Drug Treatment"],"prefix":"10.1128","volume":"62","author":[{"given":"Sanchita","family":"Das","sequence":"first","affiliation":[{"name":"Cell Death and Differentiation Research, National Institute of Immunology, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sagnik","family":"Giri","sequence":"additional","affiliation":[{"name":"Cell Death and Differentiation Research, National Institute of Immunology, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam","family":"Sundar","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrima","family":"Shaha","sequence":"additional","affiliation":[{"name":"Cell Death and Differentiation Research, National Institute of Immunology, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.16943\/ptinsa\/2015\/v81i3\/48223"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.293-305.2005"},{"key":"e_1_3_2_4_2","first-page":"399","article-title":"Current scenario of drug development for leishmaniasis","volume":"123","author":"Croft SL","year":"2006","unstructured":"Croft SL, Seifert K, Yardley V. 2006. 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