{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:09:31Z","timestamp":1792289371833,"version":"4.4.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study aimed to investigate the activity of a combination of topical paromomycin gel and oral miltefosine for the treatment of experimental cutaneous leishmaniasis caused by\n            <jats:italic>Leishmania<\/jats:italic>\n            (\n            <jats:italic>Leishmania<\/jats:italic>\n            )\n            <jats:italic>amazonensis<\/jats:italic>\n            . The efficacy of the combination, evaluated by measuring lesion size and parasite burden in the skin and spleen, was assessed in BALB\/c mice infected by\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:italic>L.<\/jats:italic>\n            )\n            <jats:italic>amazonensis<\/jats:italic>\n            . The miltefosine was administered orally at 10 mg\/kg of body weight\/day for 10 days, while 10% paromomycin gel was applied topically twice a day for 20 days. Treatment of the experimentally infected animals with a topical paromomycin-oral miltefosine combination induced a statistically significant reduction in lesion size and parasite burden in the skin and spleen, with complete healing of ulcers, compared with those treated with a placebo group. A combination of topical paromomycin gel and oral miltefosine provided enhanced efficacy in the treatment of\n            <jats:italic>L.<\/jats:italic>\n            (\n            <jats:italic>L.<\/jats:italic>\n            )\n            <jats:italic>amazonensis<\/jats:italic>\n            -infected mice, showing activity higher than that observed for the monotherapeutic regimens.\n          <\/jats:p>","DOI":"10.1128\/aac.00809-10","type":"journal-article","created":{"date-parts":[[2010,8,17]],"date-time":"2010-08-17T04:29:48Z","timestamp":1282019388000},"page":"4699-4704","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Reductions in Skin and Systemic Parasite Burdens as a Combined Effect of Topical Paromomycin and Oral Miltefosine Treatment of Mice Experimentally Infected with\n            <i>Leishmania<\/i>\n            (\n            <i>Leishmania<\/i>\n            )\n            <i>amazonensis<\/i>"],"prefix":"10.1128","volume":"54","author":[{"given":"Marta Gontijo","family":"Aguiar","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics"},{"name":"Newton Paiva University Center, Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Ma\u0301rcia Machado","family":"Pereira","sequence":"additional","affiliation":[{"name":"Newton Paiva University Center, Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University Minas Gerais, Av. Anto\u0302nio Carlos, 6627, Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Antonio Miranda","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp365"},{"key":"e_1_3_2_3_2","first-page":"5","volume":"5","year":"1987","unstructured":"Barral-Netto, M., M. S. A. Cardoso, and A. Barral. 1987. Different disease patterns in two inbred mouse strains infected with a clone of Leishmania mexicana amazonensis. 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