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Particularly, ST131 clade C1 is the most predominant clade in Japan, harboring\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              at a high frequency. However, the process of resistance gene acquisition and spread remains unclear. Here, we performed whole-genome sequencing of 19\n              <jats:italic>E. coli<\/jats:italic>\n              strains belonging to 12 STs and 12\n              <jats:italic>fimH<\/jats:italic>\n              types collected between 1997 and 2016. Additionally, we analyzed the full-length genome sequences of 96 ST131-\n              <jats:italic>H<\/jats:italic>\n              30 clade C0 and C1 strains, including those obtained from this study and those registered in public databases, to understand how ST131 clade C1 acquired and spread\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              . We detected conjugative IncFII plasmids and IncB\/O\/K\/Z plasmids carrying\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              in diverse genetic lineages of\n              <jats:italic>E. coli<\/jats:italic>\n              strains from the 1990s to the 2010s, suggesting that these plasmids played an important role in the spread of\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              . Molecular phylogenetic and molecular clock analyses of the 96 ST131-\n              <jats:italic>H<\/jats:italic>\n              30 clade C0 and C1 strains identified 8 subclades. Strains harboring\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              were clustered in subclades 4 and 5, and it was inferred that clade C1 acquired\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              around 1993. All 34 strains belonging to subclade 5 possessed\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              with IS\n              <jats:italic>Ecp1<\/jats:italic>\n              upstream at the same chromosomal position, indicating their common ancestor acquired\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              in a single IS\n              <jats:italic>Ecp1<\/jats:italic>\n              -mediated transposition event during the early formation of the subclade around 1999. Therefore, both the horizontal transfer of plasmids carrying\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              to diverse genetic lineages and chromosomal integration in the predominant genetic lineage have contributed to the spread of\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-14<\/jats:sub>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.00817-24","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T13:01:30Z","timestamp":1723467690000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Plasmid-mediated acquisition and chromosomal integration of\n            <i>bla<\/i>\n            <sub>CTX-M-14<\/sub>\n            in a subclade of\n            <i>Escherichia coli<\/i>\n            ST131-\n            <i>H<\/i>\n            30 clade C1"],"prefix":"10.1128","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9659-3186","authenticated-orcid":false,"given":"Kohji","family":"Komori","sequence":"first","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9237-1217","authenticated-orcid":true,"given":"Kotaro","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3073-6564","authenticated-orcid":false,"given":"Sohei","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-4648","authenticated-orcid":true,"given":"Yoshikazu","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University Graduate School of Medicine, Tokyo, Japan"},{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, Japan"},{"name":"Center for the Planetary Health and Innovation Science (PHIS), The IDEC Institute, Hiroshima University, Higashi-Hiroshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Tateda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Toho University Graduate School of Medicine, Tokyo, Japan"},{"name":"Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, 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