{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T17:48:47Z","timestamp":1769276927025,"version":"3.49.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Drug-Resistant Tuberculosis Research Fund, Siriraj Foundation, Faculty of Medicine Siriraj Hospital, Mahidol University"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            New fluoroquinolones (FQs) have been shown to be more active against drug-resistant\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            strains than early FQs, such as ofloxacin. Sitafloxacin (STFX) is a new fluoroquinolone with\n            <jats:italic>in vitro<\/jats:italic>\n            activity against a broad range of bacteria, including\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            . This study aimed to determine the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of STFX against all groups of drug-resistant strains, including multidrug-resistant\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            (MDR\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            ), MDR\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            with quinolone resistance (pre-XDR), and extensively drug-resistant (XDR) strains. A total of 374 drug-resistant\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            strains were tested for drug susceptibility by the conventional proportion method, and 95 strains were randomly submitted for MIC determination using the microplate alamarBlue assay (MABA). The results revealed that all the drug-resistant strains were susceptible to STFX at a critical concentration of 2 \u03bcg\/ml. Determination of the MIC\n            <jats:sub>90<\/jats:sub>\n            s of the strains showed different MIC levels; MDR\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            strains had a MIC\n            <jats:sub>90<\/jats:sub>\n            of 0.0625 \u03bcg\/ml, whereas pre-XDR and XDR\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            strains had identical MIC\n            <jats:sub>90<\/jats:sub>\n            s of 0.5 \u03bcg\/ml. Common mutations within the quinolone resistance-determining region (QRDR) of\n            <jats:italic>gyrA<\/jats:italic>\n            and\/or\n            <jats:italic>gyrB<\/jats:italic>\n            did not confer resistance to STFX, except that double mutations of GyrA at Ala90Val and Asp94Ala were found in strains with a MIC of 1.0 \u03bcg\/ml. The results indicated that STFX had potent\n            <jats:italic>in vitro<\/jats:italic>\n            activity against all the groups of drug-resistant\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            strains and should be considered a new repurposed drug for treatment of multidrug-resistant and extensively drug-resistant TB.\n          <\/jats:p>","DOI":"10.1128\/aac.00825-17","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:40:25Z","timestamp":1508805625000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["<i>In Vitro<\/i>\n            Activity and MIC of Sitafloxacin against Multidrug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis Isolated in Thailand"],"prefix":"10.1128","volume":"62","author":[{"given":"Manoon","family":"Leechawengwongs","sequence":"first","affiliation":[{"name":"Vichaiyut Hospital, Bangkok, Thailand"},{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Therdsak","family":"Prammananan","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Ministry of Science and Technology, Pathumthani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarinya","family":"Jaitrong","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Ministry of Science and Technology, Pathumthani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamaree","family":"Billamas","sequence":"additional","affiliation":[{"name":"National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Ministry of Science and Technology, Pathumthani, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nampueng","family":"Makhao","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nongnard","family":"Thamnongdee","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arirat","family":"Thanormchat","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arisa","family":"Phurattanakornkul","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somcharn","family":"Rattanarangsee","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chate","family":"Ratanajaraya","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areeya","family":"Disratthakit","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angkana","family":"Chaiprasert","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand"},{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"WHO\/HTM\/TB\/2016.13","author":"World Health Organization","year":"2016","unstructured":"World Health Organization. 2016. 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BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT. Nucleic Acids Symp Ser 41:95\u201398.","journal-title":"Nucleic Acids Symp Ser"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00825-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00825-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:06:25Z","timestamp":1645477585000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00825-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1128\/AAC.00825-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.00825-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"2017-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00825-17"}}