{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:08:04Z","timestamp":1790644084709,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The activity of the new cephalosporin CXA-101 (CXA), previously designated FR264205, was evaluated against a collection of 236 carbapenem-resistant\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            isolates, including 165 different clonal types, from a Spanish multicenter (127-hospital) study. The MICs of CXA were compared to the susceptibility results for antipseudomonal penicillins, cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones. The MIC of CXA in combination with tazobactam (4 and 8 \u03bcg\/ml) was determined for strains with high CXA MICs. The presence of acquired \u03b2-lactamases was investigated by isoelectric focusing and PCR amplification followed by sequencing. Additional \u03b2-lactamase genes were identified by cloning and sequencing. The CXA MIC\n            <jats:sub>50<\/jats:sub>\n            \/MIC\n            <jats:sub>90<\/jats:sub>\n            for the complete collection of carbapenem-resistant\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            isolates was 1\/4 \u03bcg\/ml, with 95.3% of the isolates showing an MIC of \u22648 \u03bcg\/ml. Cross-resistance with any of the antibiotics tested was not observed; the MIC\n            <jats:sub>50<\/jats:sub>\n            \/MIC\n            <jats:sub>90<\/jats:sub>\n            of CXA-101 was still 1\/4 when multidrug-resistant (MDR) strains (42% of all tested isolates) or AmpC-hyperproducing clones (53%) were analyzed. Almost all (10\/11) of the strains showing a CXA MIC of &gt;8 \u03bcg\/ml produced a horizontally acquired \u03b2-lactamase, including the metallo-\u03b2-lactamase (MBL) VIM-2 (one strain), the extended-spectrum \u03b2-lactamase (ESBL) PER-1 (one strain), several extended-spectrum OXA enzymes (OXA-101 [one strain], OXA-17 [two strains], and a newly described OXA-2 derivative [W159R] designated OXA-144 [four strains]), and a new BEL variant (BEL-3) ESBL (one strain), as identified by cloning and sequencing. Synergy with tazobactam in these 11 strains was limited, although 8 \u03bcg\/ml reduced the mean CXA MIC by 2-fold. CXA is highly active against carbapenem-resistant\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            isolates, including MDR strains. Resistance was restricted to still-uncommon strains producing an acquired MBL or ESBL.\n          <\/jats:p>","DOI":"10.1128\/aac.00834-09","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T03:44:44Z","timestamp":1259034284000},"page":"846-851","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Activity of a New Antipseudomonal Cephalosporin, CXA-101 (FR264205), against Carbapenem-Resistant and Multidrug-Resistant\n            <i>Pseudomonas aeruginosa<\/i>\n            Clinical Strains"],"prefix":"10.1128","volume":"54","author":[{"given":"Carlos","family":"Juan","sequence":"first","affiliation":[{"name":"Servicio de Microbiologi\u0301a and Unidad de Investigacio\u0301n, Hospital Son Dureta, Instituto Universitario de Investigacio\u0301n en Ciencias de la Salud, Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Zamorano","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a and Unidad de Investigacio\u0301n, Hospital Son Dureta, Instituto Universitario de Investigacio\u0301n en Ciencias de la Salud, Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 L.","family":"Pe\u0301rez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a and Unidad de Investigacio\u0301n, Hospital Son Dureta, Instituto Universitario de Investigacio\u0301n en Ciencias de la Salud, Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yigong","family":"Ge","sequence":"additional","affiliation":[{"name":"Calixa Therapeutics, San Diego, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Oliver","sequence":"additional","affiliation":[{"name":"Servicio de Microbiologi\u0301a and Unidad de Investigacio\u0301n, Hospital Son Dureta, Instituto Universitario de Investigacio\u0301n en Ciencias de la Salud, Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Antimicrob. 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