{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:06:42Z","timestamp":1790712402562,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI090155"],"award-info":[{"award-number":["R01AI090155"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,8,20]]},"abstract":"<jats:p>\n            Combating plasmid-mediated carbapenem resistance is essential to control and prevent the dissemination of carbapenem-resistant\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            (CRE). Here, we conducted a proof-of-concept study to demonstrate that CRISPR-Cas9-mediated resistance gene and plasmid curing can effectively resensitize CRE to carbapenems. A novel CRISPR-Cas9-mediated plasmid-curing system (pCasCure) was developed and electrotransferred into various clinical CRE isolates. The results showed that pCasCure can effectively cure\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            ,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NDM<\/jats:sub>\n            , and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-48<\/jats:sub>\n            in various\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            species of\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Enterobacter hormaechei<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Enterobacter xiangfangensis<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Serratia marcescens<\/jats:named-content>\n            clinical isolates, with\u2009a &gt;94% curing efficiency.\n          <\/jats:p>","DOI":"10.1128\/aac.00843-20","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T13:25:29Z","timestamp":1593782729000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["CRISPR-Cas9-Mediated Carbapenemase Gene and Plasmid Curing in Carbapenem-Resistant\n            <i>Enterobacteriaceae<\/i>"],"prefix":"10.1128","volume":"64","author":[{"given":"Mingju","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhang","family":"He","sequence":"additional","affiliation":[{"name":"National Risk Assessment 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China"},{"name":"Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China"},{"name":"Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4209-7555","authenticated-orcid":false,"given":"Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China"},{"name":"Guangdong Provincial Key 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