{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:45:36Z","timestamp":1787755536296,"version":"build-2784847793"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Among 235 extended-spectrum \u03b2-lactamase-producing\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            (ESBL) isolates collected from a nationwide surveillance performed in Taiwan, 102 (43.4%) were resistant to amikacin. Ninety-two of these 102 (90.2%) isolates were carrying CTX-M-type \u03b2-lactamases individually or concomitantly with SHV-type or CMY-2 \u03b2-lactamases. The\n            <jats:italic>armA<\/jats:italic>\n            and\n            <jats:italic>rmtB<\/jats:italic>\n            alleles were individually detected in 44 and 37 of these 92 isolates, respectively. One isolate contained both\n            <jats:italic>armA<\/jats:italic>\n            and\n            <jats:italic>rmtB<\/jats:italic>\n            . The coexistence of the\n            <jats:italic>aac(6\u2032)-Il<\/jats:italic>\n            and\n            <jats:italic>rmtB<\/jats:italic>\n            genes was detected in three isolates. CTX-M-type \u03b2-lactamase genes belonging to either group 1 (CTX-M-3 and CTX-M-15) or group 9 (CTX-M-14) were found in all\n            <jats:italic>armA-<\/jats:italic>\n            or\n            <jats:italic>rmtB<\/jats:italic>\n            -bearing ESBL-producing\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            isolates, and all were conjugatively transferable. All except one of the isolates bearing\n            <jats:italic>armA<\/jats:italic>\n            produced CTX-M enzymes of group 1, and the remaining isolate bearing\n            <jats:italic>armA<\/jats:italic>\n            produced a group 9 CTX-M-type \u03b2-lactamase. On the contrary, in the majority of\n            <jats:italic>rmtB<\/jats:italic>\n            carriers, the CTX-M-type \u03b2-lactamase belonged to group 9 (62.2%). Molecular typing revealed that the amikacin-resistant ESBL-producing\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            isolates were epidemiologically unrelated, indicating that the acquisition of resistance was not through the spread of a resistant clone or a resistance plasmid. A tandem repeat or multiple copies of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-3<\/jats:sub>\n            were found in some\n            <jats:italic>armA<\/jats:italic>\n            -bearing isolates. An IS\n            <jats:italic>Ecp1<\/jats:italic>\n            insert was found in all CTX-M ESBL-producing\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            isolates carrying\n            <jats:italic>armA<\/jats:italic>\n            or\n            <jats:italic>rmtB<\/jats:italic>\n            . In conclusion, the concomitant presence of a 16S rRNA methylase gene (\n            <jats:italic>armA<\/jats:italic>\n            or\n            <jats:italic>rmtB<\/jats:italic>\n            ) and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M<\/jats:sub>\n            among amikacin-resistant ESBL-producing\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            isolates is widespread in Taiwan.\n          <\/jats:p>","DOI":"10.1128\/aac.00852-08","type":"journal-article","created":{"date-parts":[[2008,10,21]],"date-time":"2008-10-21T00:44:44Z","timestamp":1224549884000},"page":"104-111","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Widespread Dissemination of Aminoglycoside Resistance Genes\n            <i>armA<\/i>\n            and\n            <i>rmtB<\/i>\n            in\n            <i>Klebsiella pneumoniae<\/i>\n            Isolates in Taiwan Producing CTX-M-Type Extended-Spectrum \u03b2-Lactamases"],"prefix":"10.1128","volume":"53","author":[{"given":"Ling","family":"Ma","sequence":"first","affiliation":[{"name":"Division of Clinical Research, National Health Research Institutes, Zhunan Town, Miaoli County"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Jan","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, National Health Research Institutes, Zhunan Town, Miaoli County"},{"name":"Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiun-Han","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Phone","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Medicine, Chutung Veterans Hospital, and National Yang-Ming University, Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Yee","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiu-Kay","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Chung","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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