{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T20:10:18Z","timestamp":1792527018551,"version":"4.5.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A carbapenem-resistant\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    strain was isolated in Brazil in 2004 in which no known carbapenemase gene was detected by PCR. Cloning experiments, followed by expression in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , gave an\n                    <jats:italic>E. coli<\/jats:italic>\n                    recombinant strain expressing a novel carbapenem-hydrolyzing class D \u03b2-lactamase (CHDL). OXA-143 showed 88% amino acid sequence identity with OXA-40, 63% identity with OXA-23, and 52% identity with OXA-58. It hydrolyzed penicillins, oxacillin, meropenem, and imipenem but not expanded-spectrum cephalosporins. The\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-143<\/jats:sub>\n                    gene was located on a ca. 30-kb plasmid. After transformation into reference strain\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    ATCC 19606, it conferred resistance to carbapenems. Analysis of the genetic environment of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-143<\/jats:sub>\n                    revealed that it was associated with neither insertion sequences nor integron structures. However, it was bracketed by similar replicase-encoding genes at both ends, suggesting acquisition through a homologous recombination process. This study identified a novel class D \u03b2-lactamase involved in carbapenem resistance in\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    . This enzyme is the first member of a novel subgroup of CHDLs whose prevalence remains to be determined.\n                  <\/jats:p>","DOI":"10.1128\/aac.00856-09","type":"journal-article","created":{"date-parts":[[2009,9,21]],"date-time":"2009-09-21T22:19:59Z","timestamp":1253571599000},"page":"5035-5038","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":209,"title":["OXA-143, a Novel Carbapenem-Hydrolyzing Class D \u03b2-Lactamase in\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1128","volume":"53","author":[{"given":"Paul G.","family":"Higgins","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine et Universite\u0301 Paris-Sud, K.-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Ho\u0302pital de Bice\u0302tre, Assistance Publique\/Ho\u0302pitaux de Paris, Faculte\u0301 de Me\u0301decine et Universite\u0301 Paris-Sud, K.-Bice\u0302tre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Seifert","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.583-588.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01351.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.5.717"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.1872-1880.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki425"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.06.001"},{"key":"e_1_3_2_8_2","unstructured":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 2007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb00894.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl481"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01919.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00292-09"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-8-79"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020007"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/44.3.309"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3198-3202.2005"},{"key":"e_1_3_2_18_2","unstructured":"Global epidemiology of carbapenem-resistant Acinetobacter baumannii 2008 2008"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01819.x"},{"key":"e_1_3_2_20_2","first-page":"800","volume":"16","year":"1994","unstructured":"Kovach, M. 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