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This means that alongside clonal expansion,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    can disseminate through plasmid- and transposon-mediated horizontal gene transfer. We applied whole genome sequencing to characterize the molecular epidemiology of 829\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    carbapenemase-producing isolates collected by the Canadian Nosocomial Infection Surveillance Program from 2010 to 2021. Using a combination of short-read and long-read sequencing, we obtained 202 complete and circular\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    -encoding plasmids. Using MOB-suite, 10 major plasmid clusters were identified from this data set which represented 87% (175\/202) of the Canadian\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    -encoding plasmids. We further estimated the genomic location of incomplete\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    -encoding contigs and predicted a plasmid cluster for 95% (603\/635) of these. We identified different patterns of carbapenemase mobilization across Canada related to different plasmid clusters, including clonal transmission of IncF-type plasmids (108\/829, 13%) in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    clonal complex 258 and novel repE(pEh60-7) plasmids (44\/829, 5%) in\n                    <jats:italic>Enterobacter hormaechei<\/jats:italic>\n                    ST316, and horizontal transmission of IncL\/M (142\/829, 17%) and IncN-type plasmids (149\/829, 18%) across multiple genera. Our findings highlight the diversity of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    genomic loci and indicate that multiple, distinct plasmid clusters have contributed to\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>KPC<\/jats:sub>\n                    spread and persistence in Canada.\n                  <\/jats:p>","DOI":"10.1128\/aac.00860-23","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T09:00:35Z","timestamp":1700125235000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Plasmid genomic epidemiology of\n                    <i>bla<\/i>\n                    <sub>KPC<\/sub>\n                    carbapenemase-producing\n                    <i>Enterobacterales<\/i>\n                    in Canada, 2010\u20132021"],"prefix":"10.1128","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7914-9198","authenticated-orcid":false,"given":"Nicole","family":"Lerminiaux","sequence":"first","affiliation":[{"name":"National Microbiology Laboratory, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robyn","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Bartoszko","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Davis","sequence":"additional","affiliation":[{"name":"QEII Health Sciences Centre, Halifax, Nova Scotia, 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