{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:38:48Z","timestamp":1790732328101,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003199","name":"France AgriMer","doi-asserted-by":"publisher","award":["2012-0388"],"award-info":[{"award-number":["2012-0388"]}],"id":[{"id":"10.13039\/501100003199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Resistance to extended-spectrum cephalosporins (ESCs) is a matter of considerable concern for public health. Here, we studied the spontaneous loss of an extended-spectrum beta-lactamase (ESBL)-encoding plasmid from a rifampin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            isolate orally inoculated into pigs under controlled conditions. Fecal samples were collected and cultured on rifampin-supplemented medium, and the resistance of the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            isolates to ESCs was studied by phenotypic tests, PCR detection of plasmid genes, and complete sequencing. The results showed that only 3 out of 353 rifampin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            isolates were ESC susceptible, and PCR and bioinformatics analysis confirmed the loss of the plasmid. These\n            <jats:italic>in vivo<\/jats:italic>\n            experiments indicate that the loss of an ESBL-encoding plasmid seems a rare event in gut microbiota.\n          <\/jats:p>","DOI":"10.1128\/aac.00864-16","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T03:04:34Z","timestamp":1470107074000},"page":"6046-6049","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Rare Spontaneous Loss of Multiresistance Gene Carrying IncI\/ST12 Plasmid in Escherichia coli in Pig Microbiota"],"prefix":"10.1128","volume":"60","author":[{"given":"Gwenaelle","family":"Mourand","sequence":"first","affiliation":[{"name":"Laboratoire de Ploufragan-Plouzan\u00e9, Agence Nationale de S\u00e9curit\u00e9 Sanitaire (Anses), Universit\u00e9 Bretagne Loire, Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Touzain","sequence":"additional","affiliation":[{"name":"Laboratoire de Ploufragan-Plouzan\u00e9, Agence Nationale de S\u00e9curit\u00e9 Sanitaire (Anses), Universit\u00e9 Bretagne Loire, Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Jouy","sequence":"additional","affiliation":[{"name":"Laboratoire de Ploufragan-Plouzan\u00e9, Agence Nationale de S\u00e9curit\u00e9 Sanitaire (Anses), Universit\u00e9 Bretagne Loire, Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mickael Alain","family":"Fleury","sequence":"additional","affiliation":[{"name":"Laboratoire de Ploufragan-Plouzan\u00e9, Agence Nationale de S\u00e9curit\u00e9 Sanitaire (Anses), Universit\u00e9 Bretagne Loire, Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erick","family":"Denamur","sequence":"additional","affiliation":[{"name":"INSERM, IAME, UMR1137, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, IAME, UMR1137, Sorbonne Paris Cit\u00e9, Paris, France"},{"name":"AP-HP, Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire, GH Paris Nord Val de Seine, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Kempf","sequence":"additional","affiliation":[{"name":"Laboratoire de Ploufragan-Plouzan\u00e9, Agence Nationale de S\u00e9curit\u00e9 Sanitaire (Anses), Universit\u00e9 Bretagne Loire, Ploufragan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00127-11"},{"key":"e_1_3_2_3_2","first-page":"129","article-title":"The maintenance of plasmid-containing organisms in populations of Escherichia coli","volume":"123","author":"Helling RB","year":"1981","unstructured":"Helling RB, Kinney T, Adams J. 1981. The maintenance of plasmid-containing organisms in populations of Escherichia coli. 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Genome sequence assembly using trace signals and additional sequence information, p 45\u201356. In Computing science and biology: proceedings of the German Conference on Bioinformatics GCB \u201899. 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