{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:23:38Z","timestamp":1775197418852,"version":"3.50.1"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Shared Health"},{"DOI":"10.13039\/100010318","name":"University of Manitoba","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010318","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,12,16]]},"abstract":"<jats:p>\n            Here, we characterize the\n            <jats:italic>fosA<\/jats:italic>\n            genes from three\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            clinical isolates recovered from Canadian patients. Each\n            <jats:italic>fosA<\/jats:italic>\n            sequence was individually overexpressed in\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            BW25113, and antimicrobial susceptibility testing was performed to assess their role in fosfomycin resistance. The findings from this study identify and functionally characterize FosA3, FosA8, and novel FosA7 members and highlight the importance of phenotypic characterization of\n            <jats:italic>fosA<\/jats:italic>\n            genes.\n          <\/jats:p>","DOI":"10.1128\/aac.00865-20","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T13:40:32Z","timestamp":1603201232000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Identification and Characterization of a Novel FosA7 Member from Fosfomycin-Resistant Escherichia coli Clinical Isolates from Canadian Hospitals"],"prefix":"10.1128","volume":"65","author":[{"given":"Kieran A.","family":"Milner","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3620-828X","authenticated-orcid":false,"given":"Denice C.","family":"Bay","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"},{"name":"Cadham Provincial Laboratory, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2880-2104","authenticated-orcid":false,"given":"Andrew","family":"Walkty","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"},{"name":"Shared Health, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Karlowsky","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"},{"name":"Shared Health, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Mulvey","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"},{"name":"Public Health Agency of Canada\u2013National Microbiology Laboratory, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meenu K.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"},{"name":"Public Health Agency of Canada\u2013National Microbiology Laboratory, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George G.","family":"Zhanel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1104429"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt027"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2017.156"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00068-15"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2082693"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2019.114962"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","unstructured":"Matthews TC Bristow FR Griffiths EJ Petkau A Adam J Dooley D Kruczkiewicz P Curatcha J Cabral J Fornika D Winsor GL Courtot M Bertelli C Roudgar A Feijao P Mabon P Enns E Thiessen J Keddy A Isaac-Renton J Gardy JL Tang P Carri\u00e7o JA Chindelevitch L Chauve C Graham MR McArthur AG Taboada EN Beiko RG Brinkman FS Hsiao WW Van Domselaar G The IRIDA Consortium. 2018. The Integrated Rapid Infectious Disease Analysis (IRIDA) platform. BioRxiv doi:10.1101\/381830.","DOI":"10.1101\/381830"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01834-09"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01403-19"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.34.10.2016"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2011.03016.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00710-18"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12366"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw177"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00410-17"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz380"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.4155\/fsoa-2018-0011"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S251360"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3213"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42381-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100050"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00865-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.00865-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:53:31Z","timestamp":1645480411000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.00865-20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,16]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12,16]]}},"alternative-id":["10.1128\/AAC.00865-20"],"URL":"https:\/\/doi.org\/10.1128\/aac.00865-20","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,16]]},"assertion":[{"value":"2020-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00865-20"}}