{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:22:48Z","timestamp":1778808168766,"version":"3.51.4"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["K08AI114883"],"award-info":[{"award-number":["K08AI114883"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI11103"],"award-info":[{"award-number":["R21AI11103"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI123747"],"award-info":[{"award-number":["R21AI123747"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We compared the\n            <jats:italic>in vitro<\/jats:italic>\n            activities of gentamicin (GEN), tobramycin (TOB), amikacin (AMK), and plazomicin (PLZ) against 13\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter<\/jats:named-content>\n            isolates possessing both\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:named-content>\n            carbapenemase and extended-spectrum \u03b2-lactamase (KPC+\/ESBL+) with activity against 8 KPC+\/ESBL\u2212, 6 KPC\u2212\/ESBL+, and 38 KPC\u2212\/ESBL\u2212 isolates. The rates of resistance to GEN and TOB were higher for KPC+\/ESBL+ (100% for both) than for KPC+\/ESBL\u2212 (25% and 38%, respectively), KPC\u2212\/ESBL+ (50% and 17%, respectively), and KPC\u2212\/ESBL\u2212 (0% and 3%, respectively) isolates. KPC+\/ESBL+ isolates were more likely than others to possess an aminoglycoside-modifying enzyme (AME) (100% versus 38%, 67%, and 5%;\n            <jats:italic>P<\/jats:italic>\n            = 0.007, 0.06, and &lt;0.0001, respectively) or multiple AMEs (100% versus 13%, 33%, and 0%, respectively;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01 for all). KPC+\/ESBL+ isolates also had a greater number of AMEs (mean of 4.6 versus 1.5, 0.9, and 0.05, respectively;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01 for all). GEN and TOB MICs were higher against isolates with &gt;1 AME than with \u22641 AME. The presence of at least 2\/3 of KPC, SHV, and TEM predicted the presence of AMEs. PLZ MICs against all isolates were \u22644 \u03bcg\/ml, regardless of KPC\/ESBL pattern or the presence of AMEs. In conclusion, GEN and TOB are limited as treatment options against KPC+ and ESBL+\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter<\/jats:named-content>\n            . PLZ may represent a valuable addition to the antimicrobial armamentarium. A full understanding of AMEs and other aminoglycoside resistance mechanisms will allow clinicians to incorporate PLZ rationally into treatment regimens. The development of molecular assays that accurately and rapidly predict antimicrobial responses among KPC- and ESBL-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacter<\/jats:named-content>\n            spp. should be a top research priority.\n          <\/jats:p>","DOI":"10.1128\/aac.00869-16","type":"journal-article","created":{"date-parts":[[2016,6,14]],"date-time":"2016-06-14T02:34:13Z","timestamp":1465871653000},"page":"5208-5214","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Association between the Presence of Aminoglycoside-Modifying Enzymes and\n            <i>In Vitro<\/i>\n            Activity of Gentamicin, Tobramycin, Amikacin, and Plazomicin against Klebsiella pneumoniae Carbapenemase- and Extended-Spectrum-\u03b2-Lactamase-Producing Enterobacter Species"],"prefix":"10.1128","volume":"60","author":[{"given":"Ghady","family":"Haidar","sequence":"first","affiliation":[{"name":"University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ammar","family":"Alkroud","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoji","family":"Cheng","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Travis M.","family":"Churilla","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryce M.","family":"Churilla","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan K.","family":"Shields","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"},{"name":"XDR Pathogen Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Doi","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"},{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelius J.","family":"Clancy","sequence":"additional","affiliation":[{"name":"University of Pittsburgh, Pittsburgh, Pennsylvania, USA"},{"name":"XDR Pathogen Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"},{"name":"Pittsburgh VA Healthcare System, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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