{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:44:22Z","timestamp":1790401462449,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013227","name":"MOHW | Health Promotion Administration, Ministry of Health and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013227","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013227","name":"MOHW | Health Promotion Administration, Ministry of Health and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,8,20]]},"abstract":"<jats:p>\n            The coronavirus (CoV) disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is a health threat worldwide. Viral main protease (M\n            <jats:sup>pro<\/jats:sup>\n            , also called 3C\u2010like protease [3CL\n            <jats:sup>pro<\/jats:sup>\n            ]) is a therapeutic target for drug discovery. Herein, we report that GC376, a broad-spectrum inhibitor targeting M\n            <jats:sup>pro<\/jats:sup>\n            in the picornavirus-like supercluster, is a potent inhibitor for the M\n            <jats:sup>pro<\/jats:sup>\n            encoded by SARS-CoV-2, with a half-maximum inhibitory concentration (IC\n            <jats:sub>50<\/jats:sub>\n            ) of 26.\n          <\/jats:p>","DOI":"10.1128\/aac.00872-20","type":"journal-article","created":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T12:57:50Z","timestamp":1594904270000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":84,"title":["Discovery of M Protease Inhibitors Encoded by SARS-CoV-2"],"prefix":"10.1128","volume":"64","author":[{"given":"Hui-Chen","family":"Hung","sequence":"first","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Yu","family":"Ke","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng-Nien","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan"},{"name":"Division of Infectious Diseases, Department of Pediatrics, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-An","family":"Kung","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral 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Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-En","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Ting","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ru","family":"Tsai","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Szu-Huei","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiow-Ju","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiunn-Horng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Animal Technology Laboratories, Agricultural Technology Research Institute (ATRI), Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing-Sin","family":"Liu","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang-Chou","family":"Sung","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4874-401X","authenticated-orcid":false,"given":"Shin-Ru","family":"Shih","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan, Taiwan"},{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiung-Tong","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8766-8681","authenticated-orcid":false,"given":"John Tsu-An","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, Taiwan"},{"name":"Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.03.004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0695-z"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-015-0422-1"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2196-x"},{"key":"e_1_3_3_7_2","volume-title":"Report of the WHO-China Joint Mission on Coronavirus Disease 2019 (COVID-19)","author":"WHO","year":"2020","unstructured":"WHO. 2020. 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