{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:33:03Z","timestamp":1792459983702,"version":"4.5.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic wound infections are associated with biofilm formation, which in turn has been correlated with drug resistance. However, the mechanism by which bacteria form biofilms in clinical environments is not clearly understood. This study was designed to investigate the biofilm formation potency of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>\n                    and the potential association of biofilm formation with genes encoding efflux pumps, quorum-sensing regulators, and outer membrane proteins. A total of 48 clinically isolated\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n                    strains, identified by enterobacterial repetitive intergenic consensus (ERIC)-PCR as types A-II, A-III, and A-IV, were analyzed. Three representative strains, which were designated\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n                    ABR2, ABR11, and ABS17, were used to evaluate antimicrobial susceptibility, biofilm inducibility, and gene transcription (\n                    <jats:italic>abaI<\/jats:italic>\n                    ,\n                    <jats:italic>adeB<\/jats:italic>\n                    ,\n                    <jats:italic>adeG<\/jats:italic>\n                    ,\n                    <jats:italic>adeJ<\/jats:italic>\n                    ,\n                    <jats:italic>carO<\/jats:italic>\n                    , and\n                    <jats:italic>ompA<\/jats:italic>\n                    ). A significant increase in the MICs of different classes of antibiotics was observed in the biofilm cells. The formation of a biofilm was significantly induced in all the representative strains exposed to levofloxacin. The levels of gene transcription varied between bacterial genotypes, antibiotics, and antibiotic concentrations. The upregulation of\n                    <jats:italic>adeG<\/jats:italic>\n                    correlated with biofilm induction. The consistent upregulation of\n                    <jats:italic>adeG<\/jats:italic>\n                    and\n                    <jats:italic>abaI<\/jats:italic>\n                    was detected in A-III-type\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n                    in response to levofloxacin and meropenem (1\/8 to 1\/2\u00d7 the MIC), conditions which resulted in the greatest extent of biofilm induction. This study demonstrates a potential role of the AdeFGH efflux pump in the synthesis and transport of autoinducer molecules during biofilm formation, suggesting a link between low-dose antimicrobial therapy and a high risk of biofilm infections caused by\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n                    . This study provides useful information for the development of antibiofilm strategies.\n                  <\/jats:p>","DOI":"10.1128\/aac.00877-15","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T09:55:09Z","timestamp":1433238909000},"page":"4817-4825","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":195,"title":["Biofilm Formation Caused by Clinical Acinetobacter baumannii Isolates Is Associated with Overexpression of the AdeFGH Efflux Pump"],"prefix":"10.1128","volume":"59","author":[{"given":"Xinlong","family":"He","sequence":"first","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Parasitic Diseases, Key Laboratory of Parasitic Disease Control and Prevention (Ministry of Health), Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenglai","family":"Yuan","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donglin","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhu","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huali","family":"Cheng","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Cao","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Parasitic Diseases, Key Laboratory of Parasitic Disease Control and Prevention (Ministry of Health), Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Wuxi, Jiangsu Province, People's Republic of China"},{"name":"Public Health Research Center, Jiangnan University, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guozhong","family":"Lu","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Wuxi, Wuxi, Jiangsu Province, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/500202"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03527.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.6.1026"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2013.07.014"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.42"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/432803"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2009.022"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/529198"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.23"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/652759"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01916.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200565020-00003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2009.02.017"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.5301\/ijao.5000027"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036696"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2010.512269"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1510307"},{"key":"e_1_3_3_20_2","first-page":"277","article-title":"Inhibition of quorum sensing and efflux pump system by trifluoromethyl ketone proton pump inhibitors","volume":"26","author":"Varga ZG","year":"2012","unstructured":"Varga ZG, Armada A, Cerca P, Amaral L, Mior Ahmad Subki MA, Savka MA, Szegedi E, Kawase M, Motohashi N, Molnar J. 2012. 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