{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:04:19Z","timestamp":1773752659394,"version":"3.50.1"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n From June to September 2011, a total of 305 ertapenem-nonsusceptible\n Enterobacteriaceae<\/jats:named-content>\n isolates (MICs of ertapenem \u2265 1 \u03bcg\/ml) were collected from 11 hospitals in different parts of Taiwan. The MICs of 12 antimicrobial agents against these isolates were determined using the broth microdilution method, and genes for carbapenemases were detected using PCR. Genotypes of isolates possessing carbapenemase genes were identified by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. The ertapenem-nonsusceptible\n Enterobacteriaceae<\/jats:named-content>\n isolates included\n Klebsiella pneumoniae<\/jats:named-content>\n (\n n<\/jats:italic>\n = 219),\n Escherichia coli<\/jats:named-content>\n (\n n<\/jats:italic>\n = 64),\n Enterobacter cloacae<\/jats:named-content>\n (\n n<\/jats:italic>\n = 15), and other species (\n n<\/jats:italic>\n = 7). Seven (2.3%) of the ertapenem-nonsusceptible\n Enterobacteriaceae<\/jats:named-content>\n isolates exhibited colistin MICs of >4 \u03bcg\/ml, and 24 (7.9%) were not susceptible to tigecycline (MICs > 2 \u03bcg\/ml). A total of 29 (9.5%) isolates carried genes encoding carbapenemases, namely,\n K. pneumoniae<\/jats:named-content>\n carbapenemase-2 (KPC-2) in 16 (7.3%) isolates of\n K. pneumoniae<\/jats:named-content>\n (KPC-2-KP) and IMP-8 in 5 (2.3%) isolates of\n K. pneumoniae<\/jats:named-content>\n , 5 (33.3%) isolates of\n E. cloacae<\/jats:named-content>\n , 1 isolate of\n E. coli<\/jats:named-content>\n , 1 isolate of\n Klebsiella oxytoca<\/jats:named-content>\n , and one isolate of\n Citrobacter freundii<\/jats:named-content>\n . The 16 KPC-2-KP isolates were isolated from patients at four different hospitals in northern Taiwan. All 16 of the KPC-2-KP isolates were susceptible to amikacin and colistin and had a similar pulsotype (pulsotype 1) and the same sequence type (sequence type 11). Infections due to KPC-2-KP mainly occurred in severely ill patients in the intensive care unit (\n n<\/jats:italic>\n = 14, 88%). Four patients with infections due to KPC-2-KP died within 14 days of hospitalization. The findings are the first to demonstrate intrahospital and interhospital dissemination of KPC-2-KP in northern Taiwan.\n <\/jats:p>","DOI":"10.1128\/aac.00878-12","type":"journal-article","created":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T15:41:37Z","timestamp":1342539697000},"page":"5016-5022","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Outbreak of Klebsiella pneumoniae Carbapenemase-2-Producing K. pneumoniae Sequence Type 11 in Taiwan in 2011"],"prefix":"10.1128","volume":"56","author":[{"given":"Chun-Ming","family":"Lee","sequence":"first","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan"},{"name":"Department of Infectious Diseases, Mackay Memorial Hospital, Taipei, Taiwan"},{"name":"Mackay Medicine, Nursing and Management College, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Hsing","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Far Eastern Memorial Hospital, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Sen","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung-Ching","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University and School of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung-Jung","family":"Mu","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng-Chih","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan"},{"name":"Department of Family Medicine, Taichung Hospital, Department of Health, Executive Yuan, Taichung, Taiwan"},{"name":"School of Medicine, Chung Shan Medical University, Taichung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Ren","family":"Hsueh","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1093\/jac\/dkh218","article-title":"In vitro susceptibility of recent antibiotic-resistant urinary pathogens to ertapenem and 12 other antibiotics","volume":"53","author":"Alhambra A","year":"2004","unstructured":"AlhambraA CuadrosJA CachoJ G\u00f3mez-Garc\u00e9sJL Al\u00f3sJI. 2004. In vitro susceptibility of recent antibiotic-resistant urinary pathogens to ertapenem and 12 other antibiotics. J. Antimicrob. Chemother. 53:1090\u20131094.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05914-11"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.165.12.1430"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.3018-3020.2005"},{"key":"e_1_3_3_6_2","first-page":"256","article-title":"Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities","volume":"58","author":"Centers for Disease Control and Prevention","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities. MMWR Morb. Mortal. Wkly. Rep. 58:256\u2013260.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_3_7_2","first-page":"1418","article-title":"Carbapenem-resistant Klebsiella pneumoniae associated with a long-term-care facility\u2014West Virginia, 2009-2011","volume":"60","author":"Centers for Disease Control and Prevention","year":"2011","unstructured":"Centers for Disease Control and Prevention. 2011. Carbapenem-resistant Klebsiella pneumoniae associated with a long-term-care facility\u2014West Virginia, 2009-2011. MMWR Morb. Mortal. Wkly. Rep. 60:1418\u20131420.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.4.1222-1227.2006"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2011.02.009"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06053-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr025"},{"key":"e_1_3_3_12_2","volume-title":"Performance standards for antimicrobial susceptibility testing (M100-S21).","author":"Clinical and Laboratory Standard Institute","year":"2011","unstructured":"Clinical and Laboratory Standard Institute. 2011. Performance standards for antimicrobial susceptibility testing (M100-S21). Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_13_2","volume-title":"Performance standards for antimicrobial susceptibility testing (M100-S22).","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial susceptibility testing (M100-S22). Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp498"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp029"},{"key":"e_1_3_3_16_2","unstructured":"European Society of Clinical Microbiology and Infectious Diseases. 2011. Clinical breakpoints. In European Committee on Antimicrobial Susceptibility Testing London United Kingdom. http:\/\/www.eucast.org\/."},{"key":"e_1_3_3_17_2","unstructured":"Food and Drug Administration. 2011. Class II special controls guidance document: antimicrobial susceptibility test (AST) systems. U.S. Department of Health and Human Services Food and Drug Administration Washington DC. http:\/\/www.fda.gov\/MedicalDevices\/DeviceRegulationandGuidance\/GuidanceDocuments\/ucm080564.htm. Accessed 22 March 2012."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/648451"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00070-11"},{"key":"e_1_3_3_20_2","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.ijantimicag.2010.12.003","article-title":"In vitro susceptibilities of clinical isolates of ertapenem-non-susceptible Enterobacteriaceae to nemonoxacin, tigecycline, fosfomycin and other antimicrobial agents","volume":"37","author":"Hsu MS","year":"2011","unstructured":"HsuMS. 2011. In vitro susceptibilities of clinical isolates of ertapenem-non-susceptible Enterobacteriaceae to nemonoxacin, tigecycline, fosfomycin and other antimicrobial agents. Int. J. Antimicrob. Agents 37:276\u2013278.","journal-title":"Int. J. Antimicrob. 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Dis."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr240"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00651-08"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/505598"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01103-07"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110655"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/592412"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq431"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-07"},{"issue":"33","key":"e_1_3_3_32_2","doi-asserted-by":"crossref","DOI":"10.2807\/ese.16.33.19944-en","article-title":"Outbreak due to a Klebsiella pneumoniae strain harbouring KPC-2 and VIM-1 in a German university hospital, July 2010 to January 2011","volume":"16","author":"Steinmann J","year":"2011","unstructured":"SteinmannJ. 2011. Outbreak due to a Klebsiella pneumoniae strain harbouring KPC-2 and VIM-1 in a German university hospital, July 2010 to January 2011. Euro Surveill. 16(33):pii=19944.","journal-title":"Euro Surveill."},{"key":"e_1_3_3_33_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jmii.2011.03.001","article-title":"Emergence and spread of multi-drug resistant organisms: think globally and act locally","volume":"44","author":"Tseng SH","year":"2011","unstructured":"TsengSH LeeCM LinTY ChangSC ChangFY. 2011. Emergence and spread of multi-drug resistant organisms: think globally and act locally. J. Microbiol. Immunol. Infect. 44:157\u2013165.","journal-title":"J. Microbiol. Immunol. 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