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Here, the genetic polymorphism in the\n              <jats:italic toggle=\"yes\">Kelch<\/jats:italic>\n              13 gene (\n              <jats:italic toggle=\"yes\">pfk13<\/jats:italic>\n              ) and its structural impact were analyzed.\n              <jats:italic toggle=\"yes\">Pf<\/jats:italic>\n              DNA was extracted from dried blood spots of symptomatic and asymptomatic individuals living in different epidemiological facets of Cameroon. The\n              <jats:italic toggle=\"yes\">pfk13<\/jats:italic>\n              gene was amplified by nested polymerase chain reaction, and amplicons were sequenced to detect single nucleotide polymorphisms (SNPs). The evolutionary history and the impact of the polymorphisms on physicochemical properties, structure, and function of the pfK13 protein were appraised using various\n              <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n              models. A total of ten SNPs were identified in this study, of which five non-synonymous SNPs have not been previously reported (L647\n              <jats:bold>\n                <jats:underline>F<\/jats:underline>\n              <\/jats:bold>\n              , D648\n              <jats:bold>\n                <jats:underline>V<\/jats:underline>\n              <\/jats:bold>\n              , N657\n              <jats:bold>\n                <jats:underline>S<\/jats:underline>\n              <\/jats:bold>\n              , K658\n              <jats:bold>\n                <jats:underline>R<\/jats:underline>\n              <\/jats:bold>\n              , and L663\n              <jats:bold>\n                <jats:underline>P<\/jats:underline>\n              <\/jats:bold>\n              ). The genetic diversity of\n              <jats:italic toggle=\"yes\">pfk13<\/jats:italic>\n              sequences was low, and the\n              <jats:italic toggle=\"yes\">pfk13<\/jats:italic>\n              gene evolved under the neutral model. Some mutations, especially L663\n              <jats:bold>\n                <jats:underline>P<\/jats:underline>\n              <\/jats:bold>\n              , appeared to affect the function and structure of the pfK13 protein. Analysis of the physicochemical properties of the Cameroonian pfK13 protein sequences revealed slight changes in the solvent-accessible surface area, isoelectric point, and hydrophobicity. The results support the ongoing use of ACTs in the study areas, given the absence of validated SNPs associated with artemisinin partial resistance. Computational findings suggest a possible deleterious effect of some novel SNPs on the pfK13 structure and\/or function.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.00884-24","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T13:01:31Z","timestamp":1744635691000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Novel\n            <i>Plasmodium falciparum Kelch13<\/i>\n            polymorphisms in Cameroon with structural and physicochemical impact"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4286-147X","authenticated-orcid":false,"given":"Loick P.","family":"Kojom Foko","sequence":"first","affiliation":[{"name":"Parasite & Host Biology, ICMR-National Institute of Malaria Research","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9443-558X","authenticated-orcid":false,"given":"Jahnvi","family":"Jakhan","sequence":"additional","affiliation":[{"name":"Parasite & Host Biology, ICMR-National Institute of Malaria Research","place":["New Delhi, India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9905-1857","authenticated-orcid":true,"given":"Geetika","family":"Narang","sequence":"additional","affiliation":[{"name":"Parasite & Host Biology, ICMR-National Institute of Malaria Research","place":["New Delhi, India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4145-9953","authenticated-orcid":false,"given":"Joseph","family":"Hawadak","sequence":"additional","affiliation":[{"name":"Parasite & Host Biology, ICMR-National Institute of Malaria Research","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3252-6552","authenticated-orcid":false,"given":"Carole E.","family":"Eboumbou Moukoko","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala","place":["Douala, Cameroon"]},{"name":"Malaria Research Unit, Centre Pasteur Cameroon","place":["Douala, Cameroon"]},{"name":"Laboratory of Parasitology, Mycology and Virology, Postgraduate Training Unit for Health Sciences, Postgraduate School for Pure and Applied Sciences, The University of Douala","place":["Douala, Cameroon"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0113-7394","authenticated-orcid":false,"given":"Vineeta","family":"Singh","sequence":"additional","affiliation":[{"name":"Parasite & Host Biology, ICMR-National Institute of Malaria Research","place":["New Delhi, India"]},{"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"World malaria report 2023. 2023. 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