{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:24:49Z","timestamp":1789514689850,"version":"build-2803163510"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aim of this study was to evaluate the activity of gamma interferon (IFN-\u03b3) when it was either adsorbed onto or loaded into albumin nanoparticles.\n            <jats:italic>Brucella abortus<\/jats:italic>\n            -infected macrophages and infected BALB\/c mice were selected as the models for testing of the therapeutic potentials of these cytokine delivery systems, in view of the well-established role of IFN-\u03b3-activated macrophages for the control of\n            <jats:italic>Brucella<\/jats:italic>\n            sp. infections. Whereas the encapsulation of IFN-\u03b3 inside the matrix of nanoparticles completely abrogated its activity, adsorbed IFN-\u03b3 increased by 0.75 log unit the bactericidal effect induced by RAW macrophages activated with free IFN-\u03b3, along with a higher level of production of nitric oxide. In infected BALB\/c-mice, IFN-\u03b3 adsorbed onto nanoparticles was also more active than free cytokine in reducing the number of bacteria in the spleens, and the effect was mediated by an increased ratio of IFN-\u03b3-secreting (Th1) to interleukin-4-secreting (Th2) cells. Overall, albumin nanoparticles would be suitable as carriers that target IFN-\u03b3 to macrophages and, thus, potentiate their therapeutic activity.\n          <\/jats:p>","DOI":"10.1128\/aac.00890-06","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T01:49:47Z","timestamp":1168652987000},"page":"1310-1314","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Gamma Interferon Loaded onto Albumin Nanoparticles: In Vitro and In Vivo Activities against\n            <i>Brucella abortus<\/i>"],"prefix":"10.1128","volume":"51","author":[{"given":"S.","family":"Segura","sequence":"first","affiliation":[{"name":"Departamento de Farmacia y Tecnologi\u0301a Farmace\u0301utica, Facultad de Farmacia, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Gamazo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a, Facultad de Medicina, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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