{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:09:58Z","timestamp":1790370598485,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptococcus uberis<\/jats:italic>\n                    is an environmental pathogen commonly causing bovine mastitis, an infection that is generally treated with penicillin G. No field case of true penicillin-resistant\n                    <jats:italic>S. uberis<\/jats:italic>\n                    (MIC &gt; 16 mg\/liter) has been described yet, but isolates presenting decreased susceptibility (MIC of 0.25 to 0.5 mg\/liter) to this drug are regularly reported to our laboratory. In this study, we demonstrated that\n                    <jats:italic>S. uberis<\/jats:italic>\n                    can readily develop penicillin resistance in laboratory-evolved mutants. The molecular mechanism of resistance (acquisition of mutations in penicillin-binding protein 1A [PBP1A], PBP2B, and PBP2X) was generally similar to that of all other penicillin-resistant streptococci described so far. In addition, it was also specific to\n                    <jats:italic>S. uberis<\/jats:italic>\n                    in that independent resistant mutants carried a unique set of seven consensus mutations, of which only one (Q\n                    <jats:sub>554<\/jats:sub>\n                    E in PBP2X) was commonly found in other streptococci. In parallel, independent isolates from bovine mastitis with different geographical origins (France, Holland, and Switzerland) and presenting a decreased susceptibility to penicillin were characterized. No mosaic PBPs were detected, but they all presented mutations identical to the one found in the laboratory-evolved mutants. This indicates that penicillin resistance development in\n                    <jats:italic>S. uberis<\/jats:italic>\n                    might follow a stringent pathway that would explain, in addition to the ecological niche of this pathogen, why naturally occurring resistances are still rare. In addition, this study shows that there is a reservoir of mutated PBPs in animals, which might be exchanged with other streptococci, such as\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    , that could potentially be transmitted to humans.\n                  <\/jats:p>","DOI":"10.1128\/aac.00915-09","type":"journal-article","created":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T21:59:16Z","timestamp":1263247156000},"page":"1140-1145","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Penicillin-Binding Protein Gene Alterations in\n                    <i>Streptococcus uberis<\/i>\n                    Isolates Presenting Decreased Susceptibility to Penicillin"],"prefix":"10.1128","volume":"54","author":[{"given":"Marisa","family":"Haenni","sequence":"first","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Aliments (AFSSA), Unite\u0301 Antibiore\u0301sistance et Virulence Bacte\u0301rienne, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Galofaro","sequence":"additional","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Aliments (AFSSA), Unite\u0301 Antibiore\u0301sistance et Virulence Bacte\u0301rienne, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Ythier","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlyse","family":"Giddey","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Majcherczyk","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Moreillon","sequence":"additional","affiliation":[{"name":"Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Madec","sequence":"additional","affiliation":[{"name":"Agence Franc\u0327aise de Se\u0301curite\u0301 Sanitaire des Aliments (AFSSA), Unite\u0301 Antibiore\u0301sistance et Virulence Bacte\u0301rienne, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Antibiotics in laboratory medicine 1996"},{"key":"e_1_3_2_3_2","unstructured":"Foodborne Pathog. 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