{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:39:05Z","timestamp":1792510745744,"version":"4.5.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Lomentospora prolificans<\/jats:italic>\n                      is a pathogenic filamentous fungus that causes disease primarily in people with severely compromised immune systems. It is pan-resistant to antifungal drugs, but the mechanism of its resistance to amphotericin B (AMB) is unknown. In this study, our goal was to investigate the mechanism of resistance to AMB of\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      . The AMB susceptibility of\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      protoplasts were measured using broth microdilution.\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      , either intact, homogenized, or fractionated, was incubated with AMB in broth. The same activity was carried out with\n                      <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                      as a control. These broths were then used to prepare microdilution plates with\n                      <jats:italic toggle=\"yes\">Saccharomyces cerevisiae<\/jats:italic>\n                      to determine the activity of the conditioned AMB. AMB was 16-fold more effective at inhibiting the growth of\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      protoplasts than conidia, but only 2-fold more effective against\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      protoplasts than conidia. Incubation of\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      hyphae with AMB in media diminished drug activity to a much greater extent than\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      , with an 8-fold greater fungal mass of the latter required to achieve the effect of the former. Homogenization and fractionation of\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      revealed that the factor inhibiting AMB activity was soluble with a mass &gt;100 kDa. DNase, trypsin, proteinase K, amyloglucosidase, SDS, and 0.22 \u03bcm filtration had no effect on the AMB resistance factor, while treatment with urea and acetonitrile inactivated it. In summary, we report a different mechanism for AMB resistance for\n                      <jats:italic toggle=\"yes\">L. prolificans<\/jats:italic>\n                      based on the existence of a substance residing in the cell wall that can eliminate the antifungal activity of AMB.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.00915-26","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:54:01Z","timestamp":1789570441000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Amphotericin B resistance in\n                    <i>Lomentospora prolificans<\/i>\n                    is associated with a soluble cell wall component"],"prefix":"10.1128","volume":"70","author":[{"given":"Nina T.","family":"Grossman","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9402-9167","authenticated-orcid":true,"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health","place":["Baltimore, USA"]},{"name":"Divison of Infectious Diseases of the Johns Hopkins School of Medicine","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02802.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2018.1514366"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802524506"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05910-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid3205.250760"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01811-10"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00020-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040321"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.05126-11"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01056-06"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01638-09"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02378-12"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.11.3394-3400.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780412331271081"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi047731m"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2003.tb03395.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01283-12"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01280-07"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05164-14"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-002-3858-7"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17040444"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myac036"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3498-3500.2005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.12.015"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D023036"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-3401989"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.500627"},{"key":"e_1_3_2_30_2","volume-title":"A standard for global application developed through the Clinical and Laboratory Standards Institute consensus process","author":"CLSI","year":"2008","unstructured":"CLSI. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard-second. In A standard for global application developed through the Clinical and Laboratory Standards Institute consensus process. Clinical and Laboratory Standards Institute."}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00915-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aac.00915-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:49:22Z","timestamp":1792507762000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aac.00915-26"}},"subtitle":[],"editor":[{"given":"Andreas H.","family":"Groll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,7]]}},"alternative-id":["10.1128\/aac.00915-26"],"URL":"https:\/\/doi.org\/10.1128\/aac.00915-26","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]},"assertion":[{"value":"2026-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00915-26"}}