{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:23:04Z","timestamp":1791087784680,"version":"4.1.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Community-associated methicillin-resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            (CA-MRSA) can cause osteomyelitis with severe sepsis and\/or local complications in which a Panton-Valentine leukocidin (PVL) role is suspected.\n            <jats:italic>In vitro<\/jats:italic>\n            sub-MIC antibiotic effects on growth and PVL production by 11 PVL\n            <jats:sup>+<\/jats:sup>\n            MRSA strains, including the major CA-MRSA clones (USA300, including the LAC strain; USA400; and USA1000), and 11 PVL\n            <jats:sup>+<\/jats:sup>\n            methicillin-susceptible\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">S. aureus<\/jats:named-content>\n            (MSSA) strains were tested in microplate culture. Time-kill analyses with ceftobiprole at its MIC were also run with LAC. Efficacies of ceftobiprole (40 mg\/kg of body weight subcutaneously [s.c.] four times a day [q.i.d.]) or vancomycin (60 mg\/kg intramuscularly [i.m.] twice a day [b.i.d.]) alone or combined with rifampin (10 mg\/kg b.i.d.) against rabbit CA-MRSA osteomyelitis, induced by tibial injection of 3.4 \u00d7 10\n            <jats:sup>7<\/jats:sup>\n            CFU of LAC, were compared. Treatment, started 14 days postinoculation, lasted 14 days.\n            <jats:italic>In vitro<\/jats:italic>\n            , 6\/11 strains cultured with sub-MICs of ceftobiprole produced 1.6- to 4.8-fold more PVL than did the controls, with no link to specific clones. Rifampin decreased PVL production by all tested strains. In time-kill analyses at the LAC MIC (0.75 mg\/liter), PVL production rose transiently at 6 and 8 h and then declined 2-fold at 16 h, concomitant with a 2-log\n            <jats:sub>10<\/jats:sub>\n            -CFU-count decrease.\n            <jats:italic>In vivo<\/jats:italic>\n            , the mean log\n            <jats:sub>10<\/jats:sub>\n            CFU\/g of bone for ceftobiprole (1.44 \u00b1 0.40) was significantly lower than that for vancomycin (2.37 \u00b1 1.22) (\n            <jats:italic>P<\/jats:italic>\n            = 0.034), with 7\/10 versus 5\/11 bones sterilized, respectively. Combination with rifampin enhanced ceftobiprole (1.16 \u00b1 0.04 CFU\/g of bone [\n            <jats:italic>P<\/jats:italic>\n            = 0.056], 11\/11 sterile bones) and vancomycin (1.23 \u00b1 0.06 CFU\/g [\n            <jats:italic>P<\/jats:italic>\n            = 0.011], 11\/11 sterile bones) efficacies. Ceftobiprole bactericidal activity and the rifampin anti-PVL effect could play a role in these findings, which should be of interest for treating CA-MRSA osteomyelitis.\n          <\/jats:p>","DOI":"10.1128\/aac.00926-12","type":"journal-article","created":{"date-parts":[[2012,10,2]],"date-time":"2012-10-02T06:20:19Z","timestamp":1349158819000},"page":"6291-6297","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Ceftobiprole Efficacy\n            <i>In Vitro<\/i>\n            against Panton-Valentine Leukocidin Production and\n            <i>In Vivo<\/i>\n            against Community-Associated Methicillin-Resistant Staphylococcus aureus Osteomyelitis in Rabbits"],"prefix":"10.1128","volume":"56","author":[{"given":"Azzam","family":"Saleh-Mghir","sequence":"first","affiliation":[{"name":"D\u00e9partement de M\u00e9decine Aigu\u00eb Sp\u00e9cialis\u00e9e, H\u00f4pital Universitaire Raymond Poincar\u00e9, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Garches"},{"name":"EA 3647, Facult\u00e9 de M\u00e9decine Paris-\u00cele-de-France Ouest, Universit\u00e9 Versailles Saint-Quentin, Versailles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oana","family":"Dumitrescu","sequence":"additional","affiliation":[{"name":"INSERM U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Lyon 1, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lien","family":"Dinh","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine Aigu\u00eb Sp\u00e9cialis\u00e9e, H\u00f4pital Universitaire Raymond Poincar\u00e9, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Garches"},{"name":"EA 3647, Facult\u00e9 de M\u00e9decine Paris-\u00cele-de-France Ouest, Universit\u00e9 Versailles Saint-Quentin, Versailles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yassine","family":"Boutrad","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine Aigu\u00eb Sp\u00e9cialis\u00e9e, H\u00f4pital Universitaire Raymond Poincar\u00e9, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Garches"},{"name":"EA 3647, Facult\u00e9 de M\u00e9decine Paris-\u00cele-de-France Ouest, Universit\u00e9 Versailles Saint-Quentin, Versailles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Massias","sequence":"additional","affiliation":[{"name":"Laboratoire de Toxicologie-Pharmacocin\u00e9tique, H\u00f4pital Bichat-Claude-Bernard, AP-HP, Universit\u00e9 Paris 7-Diderot, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9milie","family":"Martin","sequence":"additional","affiliation":[{"name":"INSERM U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Lyon 1, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Vandenesch","sequence":"additional","affiliation":[{"name":"INSERM U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Lyon 1, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Etienne","sequence":"additional","affiliation":[{"name":"INSERM U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Lyon 1, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Lina","sequence":"additional","affiliation":[{"name":"INSERM U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Facult\u00e9 de M\u00e9decine Lyon Est, Universit\u00e9 Lyon 1, Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Claude","family":"Cr\u00e9mieux","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine Aigu\u00eb Sp\u00e9cialis\u00e9e, H\u00f4pital Universitaire Raymond Poincar\u00e9, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Garches"},{"name":"EA 3647, Facult\u00e9 de M\u00e9decine Paris-\u00cele-de-France Ouest, Universit\u00e9 Versailles Saint-Quentin, Versailles"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02105.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2005-0566"},{"key":"e_1_3_2_4_2","first-page":"325","article-title":"Severe methicillin-resistant Staphylococcus aureus community-acquired pneumonia associated with influenza\u2014Louisiana and Georgia, December 2006\u2013January 2007","volume":"56","author":"Centers for Disease Control and Prevention","year":"2007","unstructured":"Centers for Disease Control and Prevention. 2007. 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